Immune signaling pathways activated in response to different pathogenic micro-organisms in Bombyx mori.

Abstract

The JAK/STAT, Toll, Imd, and RNAi pathways are the major signaling pathways associated with insect innate immunity. To explore the different immune signaling pathways triggered in response to pathogenic micro-organism infections in the silkworm, Bombyx mori, the expression levels of the signal transducer and activator of transcription (BmSTAT), spatzle-1 (Bmspz-1), peptidoglycan-recognition protein LB (BmPGRP-LB), peptidoglycan-recognition protein LE (BmPGRP-LE), argonaute 2 (Bmago2), and dicer-2 (Bmdcr2) genes after challenge with Escherichia coli (E. coli), Serratiamarcescens (Sm), Bacillus bombyseptieus (Bab), Beauveriabassiana (Beb), nucleopolyhedrovirus (BmNPV), cypovirus (BmCPV), bidensovirus (BmBDV), or Nosemabombycis (Nb) were determined using real-time PCR. We found that the JAK/STAT pathway could be activated by challenge with BmNPV and BmBDV, the Toll pathway could be most robustly induced by challenge with Beb, the Imd pathway was mainly activated in response to infection by E. coli and Sm, and the RNAi pathway was not activated by viral infection, but could be triggered by some bacterial infections. These findings yield insights into the immune signaling pathways activated in response to different pathogenic micro-organisms in the silkworm.

• Authors: Wei Liu, Jiabin Liu, Yahong Lu, Yongchang Gong, Min Zhu, Fei Chen, Zi Liang, Liyuan Zhu, Sulan Kuang, Xiaolong Hu, Guangli Cao, Renyu Xue, Chengliang Gong
• Journal: Molecular immunology (Mol Immunol) Vol. 65 Issue 2 Pg. 391-7 (Jun 2015) ISSN: 1872-9142 [Electronic] England
• PMID: 25745806 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Insect Proteins

Topics

• Animals
• Bacteria (immunology)
• Bacterial Infections (immunology)
• Bombyx (immunology, microbiology, virology)
• Insect Proteins (immunology)
• Insect Viruses (immunology)
• Microsporida (immunology)
• Microsporidiosis (immunology)
• Signal Transduction (immunology)
• Virus Diseases (immunology)