Third-degree hindpaw burn injury induced apoptosis of lumbar spinal cord ventral horn motor neurons and sciatic nerve and muscle atrophy in rats.

Abstract

BACKGROUND:

Severe burns result in hypercatabolic state and concomitant muscle atrophy that persists for several months, thereby limiting patient recovery. However, the effects of burns on the corresponding spinal dermatome remain unknown. This study aimed to investigate whether burns induce apoptosis of spinal cord ventral horn motor neurons (VHMNs) and consequently cause skeletal muscle wasting.

METHODS:

Third-degree hindpaw burn injury with 1% total body surface area (TBSA) rats were euthanized 4 and 8 weeks after burn injury. The apoptosis profiles in the ventral horns of the lumbar spinal cords, sciatic nerves, and gastrocnemius muscles were examined. The Schwann cells in the sciatic nerve were marked with S100. The gastrocnemius muscles were harvested to measure the denervation atrophy.

RESULT:

The VHMNs apoptosis in the spinal cord was observed after inducing third-degree burns in the hindpaw. The S100 and TUNEL double-positive cells in the sciatic nerve increased significantly after the burn injury. Gastrocnemius muscle apoptosis and denervation atrophy area increased significantly after the burn injury.

CONCLUSION:

Local hindpaw burn induces apoptosis in VHMNs and Schwann cells in sciatic nerve, which causes corresponding gastrocnemius muscle denervation atrophy. Our results provided an animal model to evaluate burn-induced muscle wasting, and elucidate the underlying mechanisms.

• Authors: Sheng-Hua Wu, Shu-Hung Huang, Kuang-I Cheng, Chee-Yin Chai, Jwu-Lai Yeh, Tai-Cheng Wu, Yi-Chiang Hsu, Aij-Lie Kwan
• Journal: BioMed research international (Biomed Res Int) Vol. 2015 Pg. 372819 ( 2015) ISSN: 2314-6141 [Electronic] United States
• PMID: 25695065 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Anterior Horn Cells (pathology)
• Apoptosis (physiology)
• Burns (pathology)
• Hindlimb (pathology)
• In Situ Nick-End Labeling (methods)
• Male
• Muscle, Skeletal (pathology)
• Muscular Atrophy (pathology)
• Rats
• Rats, Sprague-Dawley
• Schwann Cells (pathology)
• Sciatic Nerve (pathology)
• Spinal Cord Ventral Horn (pathology)