Ischemia-induced changes in myocardial paramagnetic metabolites: implications for intracellular oxy-radical generation.

Abstract

Evidence to identify the cellular sources of oxy-radical generation in myocardium has been of an indirect nature. We have used low-temperature ESR spectroscopy to identify and characterize ischemia-induced changes in myocardial paramagnetic metabolites. Iron-sulfur proteins associated with the NADH or succinate dehydrogenases of the mitochondrial electron-transport chain were progressively reduced with the onset and development of ischemia. This study provides direct evidence for ischemia-induced changes in an intracellular source of superoxide radical generation that may contribute to oxy-radical production during reperfusion.

• Authors: J E Baker, B Kalyanaraman
• Journal: FEBS letters (FEBS Lett) Vol. 244 Issue 2 Pg. 311-4 (Feb 27 1989) ISSN: 0014-5793 [Print] England
• PMID: 2537756 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Free Radicals

Topics

• Animals
• Coronary Disease (metabolism)
• Electron Spin Resonance Spectroscopy (methods)
• Free Radicals
• In Vitro Techniques
• Models, Biological
• Myocardium (metabolism)
• Perfusion
• Rats
• Rats, Inbred Strains