[Change of Bt protein in soil after growing Bt corns and returning corn straws to soil and its effects on soil nutrients].

Abstract

The spatiotemporal dynamics of Bt protein in soil and the change of soil nutrients in rhizosphere soil, root surface soil and soils at 0-20, 20-40 and 40-60 cm were measured in greenhouse experiments. Two Bt corns, 5422Bt1 and 5422CBCL, and their near isogenic non-Bt variety 5422 were grown for 90 days and the crop residues were retained to soil. Results showed that 1.59 and 2.78 ng x g(-1) Bt protein were detected in the rhizosphere soil with Bt corns 5422Bt1 and 5422CBCL immediately after harvest. However, there were only trace amounts of Bt protein (< 0.5 ng x g(-1)) were detected in root surface soil after 90 days and in bulk soil in the two Bt corn treatments after 30, 60 and 90 days. When corn residues returned to soil, Bt protein declined rapidly within 3 days and only trace amounts of Bt protein were measured after 7 days. There were no sig- nificant differences in organic matter, available nutrient (alkaline hydrolytic N, available P, available K) or total nutrient (total N, total P, total K) in root surface soils and soils at 0-20 cm, 20-40 cm and 40-60 cm among the Bt and non-Bt corns after 90 days. Sixty days after returning crop residues of 5422Btl to soil, the contents of organic matter and total N increased and the content of available K reduced significantly in the 0-20 cm soil depth. There were no significant differences in any other parameter at the 0-20 cm depth, neither for any parameter in the 20-40 cm and 40-60 cm soil depths compared to those in the non-Bt corn 5422 treatment. There were no significant differences in soil nutrient contents in Bt corn 5422CBCL treatment compared to those in non-Bt corn 5422 treatment except that available phosphorus content was reduced in root surface soils, and total P content increased at the 0-20 cm soil depth after 90 days. When crop residues of Bt corn 5422 CBCL were returned to soil, only available P content in the 0-20 cm soil layer was evidently higher compared to the soil receiving crop residues of non-Bt corn 5422. Results suggested that Bt protein released from root and crop residues of Bt corns would not accumulate in soil, and growing Bt corns and returning crop residues to soil would have no significant effect on soil nutrients in general.

• Authors: Ping Zeng, Yuan-Jiao Feng, Wan-Chun Zhang, Yan-Fei Zhang, Wen-Chao Dong, Jian-Wu Wang
• Journal: Ying yong sheng tai xue bao = The journal of applied ecology (Ying Yong Sheng Tai Xue Bao) Vol. 25 Issue 7 Pg. 1997-2003 (Jul 2014) ISSN: 1001-9332 [Print] China
• PMID: 25345050 (Publication Type: Journal Article)

Chemical References

• Bacillus thuringiensis Toxins
• Bacterial Proteins
• Endotoxins
• Hemolysin Proteins
• Soil
• insecticidal crystal protein, Bacillus Thuringiensis
• Phosphorus
• Nitrogen
• Potassium

Topics

• Bacillus thuringiensis Toxins
• Bacterial Proteins (chemistry)
• Endotoxins (chemistry)
• Hemolysin Proteins (chemistry)
• Nitrogen (chemistry)
• Phosphorus (chemistry)
• Plant Roots
• Plants, Genetically Modified (chemistry)
• Potassium (chemistry)
• Rhizosphere
• Soil (chemistry)
• Zea mays (chemistry, genetics)