[Clinical significance of TET2 gene expression in 157 adult acute myeloid leukemia patients with normal cytogenetics].

Abstract

OBJECTIVE:

To explore the clinical significance of ten-eleven-translocation methylcytosine dioxygenase 2 (TET2) mRNA expression levels in adult acute myeloid leukemia patients with normal cytogenetics (CN-AML).

METHODS:

Expression levels of TET2 mRNA were measured by real-time PCR in 157 adult CN-AML, and its clinical impact in CN-AML was evaluated as well.

RESULTS:

TET2 gene expression levels from bone marrow mononuclear cells (BMMNCs) [7.29(3.41-9.99)] and CD34+ cells [6.02(5.64-6.54)] in CN-AML were significantly lower than those [BMMNCs: 8.13(6.68-9.04), P=0.026; CD34+ cells: 6.48(5.97-7.12), P=0.034] in healthy control. And TET2 mRNA level at diagnosis [7.32(6.11-8.41)] was obviously lower than that at complete remission [8.39(7.76-8.79), P<0.01]. CN-AML patients with lower levels of TET2 mRNA showed worse survival rate [(32.7±5.9)%] at 18-month than those with higher levels [(48.6±6.9)%, P=0.041]. In multivariate analysis, lower level of TET2 mRNA was an independent prognostic factor for OS [hazard ratio(HR)2.032, 95% confidence interval (CI)1.272-3.247, P=0.003] and event-free survival [HR 1.532, 95% CI 1.014-2.314, P=0.043].

CONCLUSION:

The level of TET2 mRNA is significantly lower in patients with CN-AML and it is an independent negative prognostic factor. TET2 could be an important factor for the molecular-based risk stratification in CN-AML.

• Authors: Zhijuan Zhu, Jian Chen, Mengxia Yu, Feifei Chen, Zhimei Chen, Jiyu Lou, Hongyan Tong, Jian Huang, Wenbin Qian, Haitao Meng, Jie Jin
• Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (Zhonghua Xue Ye Xue Za Zhi) Vol. 35 Issue 9 Pg. 802-7 (Sep 2014) ISSN: 0253-2727 [Print] China
• PMID: 25246247 (Publication Type: Journal Article)

Chemical References

• DNA-Binding Proteins
• Proto-Oncogene Proteins
• Dioxygenases
• TET2 protein, human

Topics

• Adult
• Cytogenetic Analysis
• Cytogenetics
• DNA-Binding Proteins (genetics)
• Dioxygenases
• Disease-Free Survival
• Gene Expression
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic
• Humans
• Leukemia, Myeloid, Acute (genetics)
• Proto-Oncogene Proteins (genetics)
• Real-Time Polymerase Chain Reaction