Abstract |
Loss of expression of beta subunit of succinate dehydrogenase (SDHB) was proved to be present in a subgroup of KIT/PDGFRA wt gastrointestinal stromal tumors (GISTs). To evaluate possible diagnostic utility of SDHB immunohistochemistry in the differential diagnostics of mesenchymal tumors of gastrointestinal tract (GIT), 11 cases of KIT/PDGFRA wt GISTs, 12 gastric schwannomas (GSs), 20 solitary fibrous tumors (SFTs), 4 leiomyomas (LMs), 16 leiomyosarcomas (LMSs), 5 synovial sarcomas (SSs), 3 endometrioid stromal sarcomas (ESSs), and 1 ileal inflammatory myofibroblastic tumor (IMT) were investigated for SDHB immunoexpression together with molecular genetic analysis of genes encoding succinate dehydrogenase (SDH). Three recent cases of KIT/PDGFRA mutant GISTs were used as controls. Among the 11 KIT/PDGFRA wt GISTs, 6 expressed SDHB, 1 of them harboring a sequence change of SDHD. All SDHB-negative cases were SDHB-D wt. In 1 of the control GIST cases molecular genetic analysis revealed an SDHD sequence change in addition to a mutation in KIT exon 11. No SFT was truly SDHB-negative, but in 2 of them the staining was impossible to analyze. Furthermore, 1 SFT carried an SDHB and another 1 SDHD sequence change. All GSs, LMs, LMSs, SSs, ESSs, and IMT were SDHB-positive or non-analyzable, and SDHB-D wt. Additional factors may play a role in regulating expression of SDHB. Furthermore, SDHB immunohistochemistry alone may be misleading in excluding tumors other than GIST (especially SFT) in the differential diagnosis of KIT/PDGFRA wt mesenchymal tumors of GIT.
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Authors | M Dubova, M Sedivcova, M Michal, B Kokoskova, A Ryska, D Smid, O Daum |
Journal | Histology and histopathology
(Histol Histopathol)
Vol. 30
Issue 2
Pg. 223-32
(Feb 2015)
ISSN: 1699-5848 [Electronic] Spain |
PMID | 25205505
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Neoplasm
- Succinate Dehydrogenase
- Proto-Oncogene Proteins c-kit
- Receptor, Platelet-Derived Growth Factor alpha
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Topics |
- Adult
- Aged
- Child
- DNA, Neoplasm
(genetics)
- Diagnosis, Differential
- Female
- Gastrointestinal Stromal Tumors
(diagnosis, enzymology, genetics)
- Humans
- Immunohistochemistry
- Male
- Mesoderm
(pathology)
- Middle Aged
- Proto-Oncogene Proteins c-kit
(genetics, metabolism)
- Receptor, Platelet-Derived Growth Factor alpha
(genetics, metabolism)
- Succinate Dehydrogenase
(genetics, metabolism)
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