Characterization and profiling of immunomodulatory genes in resident mesenchymal stem cells reflect the Th1-Th17/Th2 imbalance of psoriasis.

Abstract

The expression of genes encoding for Th1, Th2 and Th17 cytokines has been extensively evaluated in differentiated skin cells of psoriatic patients. The microenvironment exerts a control on the phenotype of resident mesenchymal stem cells (MSCs) into the skin of psoriasis patients. Aim of the study was to extensively evaluate the relative expression of 43 genes encoding for Th1, Th2 and Th17 cytokines in MSCs isolated from skin of psoriasis patients. MSCs resident into psoriatic skin were isolated, characterized and profiled by PCR array for the relative expression of genes encoding for cytokines involved in Th1, Th2 and Th17 pathways. MSCs isolated from the skin of healthy subjects were used as control. The MSCs isolated from skin of psoriasis patients showed a greater relative expression of the most part of the analyzed genes encoding for Th1 and Th17 cytokines: INF-γ, CCR5, CXCL9, CXCL10, IL6, IL8, TNF-α, IL23A, CCL2, CCL20, CXCL2, CXCL5, IL17C, IL17F, IL17RA, IL21, TLR2 than healthy subjects. On the contrary, the relative expression of genes encoding for Th2 cytokines: CCL1, CCL22, CXCL12, IL2, IL3, IL4, IL13B, IL 22, IL 27, TGF-β1, was similar between the MSCs isolated from psoriasis and healthy subjects. In conclusion, the MSCs isolated from psoriasis show an imbalance between the Th1-Th17 and Th2 pathways, which reflects the well-known abnormal balance observed in differentiated skin cells. This evidence could strengthen the hypothesis of an early involvement of resident MSCs in the pathogenesis of psoriasis.

• Authors: A Campanati, M Orciani, V Consales, R Lazzarini, G Ganzetti, G Di Benedetto, R Di Primio, A Offidani
• Journal: Archives of dermatological research (Arch Dermatol Res) Vol. 306 Issue 10 Pg. 915-20 (Dec 2014) ISSN: 1432-069X [Electronic] Germany
• PMID: 25160906 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines

Topics

• Adult
• Aged
• Case-Control Studies
• Cells, Cultured
• Cytokines (genetics, metabolism)
• Female
• Gene Expression Profiling (methods)
• Gene Expression Regulation
• Humans
• Male
• Mesenchymal Stem Cells (immunology, metabolism)
• Middle Aged
• Oligonucleotide Array Sequence Analysis
• Phenotype
• Prospective Studies
• Psoriasis (genetics, immunology, metabolism)
• Real-Time Polymerase Chain Reaction
• Skin (immunology, metabolism)
• Th1 Cells (immunology, metabolism)
• Th1-Th2 Balance
• Th17 Cells (immunology, metabolism)
• Th2 Cells (immunology, metabolism)