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Molecular typing of Treponema pallidum in the Czech Republic during 2011 to 2013: increased prevalence of identified genotypes and of isolates with macrolide resistance.

Abstract
From January 2011 to December 2013, a total of 262 samples, from 188 patients suspected of having syphilis were tested for the presence of treponemal DNA by PCR amplification of five chromosomal loci, including the polA (TP0105), tmpC (TP0319), TP0136, TP0548, and 23S rRNA genes. Altogether, 146 samples from 103 patients were PCR positive for treponemal DNA. A set of 81 samples from 62 PCR-positive patients were typeable, and among them, nine different genotypes were identified. Compared to a previous study in the Czech Republic during 2004 to 2010, the number of genotypes detected among syphilis patients in a particular year increased to six in both 2012 and 2013, although they were not the same six. The proportion of macrolide-resistant clinical isolates in this 3-year study was 66.7%.
AuthorsLinda Grillová, Helena Pĕtrošová, Lenka Mikalová, Radim Strnadel, Eliška Dastychová, Ivana Kuklová, Martina Kojanová, Miluše Kreidlová, Daniela Vaňousová, Jana Hercogová, Přemysl Procházka, Hana Zákoucká, Alena Krchňáková, Vladimír Vašků, David Šmajs
JournalJournal of clinical microbiology (J Clin Microbiol) Vol. 52 Issue 10 Pg. 3693-700 (Oct 2014) ISSN: 1098-660X [Electronic] United States
PMID25100820 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2014, American Society for Microbiology. All Rights Reserved.
Chemical References
  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Macrolides
  • RNA, Ribosomal, 23S
Topics
  • Adult
  • Anti-Bacterial Agents (pharmacology)
  • Czech Republic (epidemiology)
  • DNA, Bacterial (chemistry, genetics)
  • Drug Resistance, Bacterial
  • Female
  • Genes, Bacterial
  • Genetic Variation
  • Genotype
  • Humans
  • Macrolides (pharmacology)
  • Male
  • Molecular Typing
  • Prevalence
  • RNA, Ribosomal, 23S (genetics)
  • Syphilis (epidemiology, microbiology)
  • Treponema pallidum (classification, drug effects, genetics)

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