Abstract | AIM: Identifying differences in plasma metabolic profiling between Lp-PLA2 279VV and 279FF in individuals without metabolic syndrome (MetS) can be used to elucidate the roles of novel Lp-PLA2 activities in normal physiological processes. METHODS: Non-MetS individuals with 279FF (n=36) and age-, sex- and BMI-matched VV subjects (n=36) were included in this analysis. RESULTS: The FF subjects exhibited no appreciable enzyme activity. No significant differences were observed between the VV and FF subjects in the serum lipid profiles or hs-CRP, plasma ox-LDL, MDA or urinary 8-epi-PGF2α levels. The FF subjects also showed lower activities of lyso- phosphatidylcholine (lysoPC) (16:0) (p=0.003) and oleamide (p<0.001) and a higher activity of L-tryptophan (p=0.016) than the VV subjects. In addition, the Lp-PLA2 activity positively correlated with the lysoPC (16:0) and lysoPC (18:0) activities and negatively correlated with the PC (16:0/22:6) and L-tryptophan activities in the VV subjects. Furthermore, in the VV subjects, the lysoPC (16:0) and lysoPC (18:0) activities negatively correlated with the presence of PCs containing 14:0/20:2, 14:0/22:4 and 16:0/22:6, while the oleamide activity exhibited a strong positive correlation with lysoPCs and a negative correlation with PCs, whereas the relationship between oleamide and lysoPCs and PCs was weaker in the FF subjects. CONCLUSIONS: The present results indicate that the natural absence of the plasma Lp-PLA2 activity due to carriage of the Lp-PLA2 279FF genotype may reduce the generation of lysoPC (16:0) and oleamide and thereby enhance the activity of plasma tryptophan in normal physiological processes.
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Authors | Saem Jung, Minjoo Kim, Jey Sook Chae, Sang-Hyun Lee, Jaehyun Joo, Jong Ho Lee |
Journal | Journal of atherosclerosis and thrombosis
(J Atheroscler Thromb)
Vol. 21
Issue 12
Pg. 1243-52
( 2014)
ISSN: 1880-3873 [Electronic] Japan |
PMID | 25078067
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Blood Glucose
- Fatty Acids, Nonesterified
- Lipids
- Lipoproteins, LDL
- oxidized low density lipoprotein
- 8-epi-prostaglandin F2alpha
- Malondialdehyde
- Tryptophan
- C-Reactive Protein
- Dinoprost
- 1-Alkyl-2-acetylglycerophosphocholine Esterase
- PLA2G7 protein, human
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Topics |
- 1-Alkyl-2-acetylglycerophosphocholine Esterase
(genetics)
- Adult
- Alleles
- Anthropometry
- Blood Glucose
(analysis, metabolism)
- Blood Pressure
- Body Mass Index
- C-Reactive Protein
(metabolism)
- Dinoprost
(analogs & derivatives, urine)
- Fatty Acids, Nonesterified
(chemistry)
- Female
- Genotype
- Humans
- Lipids
(blood)
- Lipoproteins, LDL
(metabolism)
- Male
- Malondialdehyde
(blood)
- Mass Spectrometry
- Metabolic Syndrome
(genetics)
- Metabolome
- Phenotype
- Risk Factors
- Tryptophan
(chemistry)
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