Abstract |
Therapeutic drug monitoring (TDM) of antiepileptic drugs (AEDs) has been recognized as a useful tool in management of epilepsy. We developed a simple analytical method for simultaneous determination of four second generation AEDs, including gabapentin (GBP), pregabalin ( PGB), vigabatrin (VGB), and topiramate (TOP). Analytes were extracted from human plasma using universal solid phase extraction, derivatized with 4-chloro-7-nitrobenzofurazan ( NBD-Cl) and analyzed by HPLC with fluorescence detection. Using mass spectrometry we confirmed that NBD-Cl reacts with sulfamate group of TOP similarly as with amine group of the other three analytes. The method is linear (r(2)>0.998) across investigated analytical ranges (0.375-30.0μg/mL for GBP, PGB, and VGB; 0.50-20.0μg/mL for TOP). Intraday and interday precision do not exceed 9.40%. The accuracy is from 95.6% to 106%. The recovery is higher than 80.6%, and the lower limit of quantification is at least 0.5μg/mL. The method is selective and robust. For TOP determination the method was compared to a previously published method and the results obtained by the two methods were in good agreement. The developed method is suitable for routine TDM.
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Authors | Boštjan Martinc, Robert Roškar, Iztok Grabnar, Tomaž Vovk |
Journal | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
(J Chromatogr B Analyt Technol Biomed Life Sci)
Vol. 962
Pg. 82-88
(Jul 01 2014)
ISSN: 1873-376X [Electronic] Netherlands |
PMID | 24907547
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Validation Study)
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Copyright | Copyright © 2014 Elsevier B.V. All rights reserved. |
Chemical References |
- Amines
- Anticonvulsants
- Cyclohexanecarboxylic Acids
- Topiramate
- Fructose
- Pregabalin
- gamma-Aminobutyric Acid
- Gabapentin
- Vigabatrin
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Topics |
- Amines
(blood)
- Anticonvulsants
(blood)
- Chromatography, High Pressure Liquid
(methods)
- Cyclohexanecarboxylic Acids
(blood)
- Drug Monitoring
(methods)
- Fluorescence
- Fructose
(analogs & derivatives, blood)
- Gabapentin
- Humans
- Limit of Detection
- Pregabalin
- Solid Phase Extraction
(methods)
- Topiramate
- Vigabatrin
(blood)
- gamma-Aminobutyric Acid
(analogs & derivatives, blood)
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