Noninvasive detection of colorectal cancer by analysis of exhaled breath.

Abstract

There has been growing interest in exhaled breath analysis for cancer screening and disease monitoring; however, limited breath biomarker information exists regarding colorectal cancer (CRC). The objective of this study was to screen for breath biomarkers of CRC. Exhaled breath was collected from 20 CRC patients and 20 healthy controls; subsequently, solid-phase microextraction-gas chromatography/mass spectrometry (SPME-GC/MS) was used to assess the exhaled volatile organic compounds (VOCs) of the study participants. The statistical methods of principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to process the final data. The VOCs in the exhalations of CRC patients exhibited significant differences from the VOCs in the exhalations of healthy controls; in particular, relative to the latter exhalations, the former exhalations contain significantly higher levels of cyclohexanone, 2,2-dimethyldecane, dodecane, 4-ethyl-1-octyn-3-ol, ethylaniline, cyclooctylmethanol, trans-2-dodecen-1-ol, and 3-hydroxy-2,4,4-trimethylpentyl 2-methylpropanoate but significantly lower levels of 6-t-butyl-2,2,9,9-tetramethyl-3,5-decadien-7-yne (P < 0.05). Analyses of breath VOCs provide a related model of CRC exhalation that could represent an effective and convenient screening method for this disease.

• Authors: Changsong Wang, Chaofu Ke, Xiaoyang Wang, Chunjie Chi, Lei Guo, Suqi Luo, Zhigang Guo, Guowang Xu, Fengmin Zhang, Enyou Li
• Journal: Analytical and bioanalytical chemistry (Anal Bioanal Chem) Vol. 406 Issue 19 Pg. 4757-63 (Jul 2014) ISSN: 1618-2650 [Electronic] Germany
• PMID: 24820062 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Volatile Organic Compounds

Topics

• Breath Tests (methods)
• Colorectal Neoplasms (diagnosis)
• Discriminant Analysis
• Female
• Gas Chromatography-Mass Spectrometry (methods)
• Humans
• Male
• Middle Aged
• Principal Component Analysis
• Solid Phase Microextraction (methods)
• Volatile Organic Compounds (analysis)