Low-level laser therapy for osteonecrotic lesions: effects on osteoblasts treated with zoledronic acid.

Abstract

PURPOSE:

Clinical studies have shown that low-level laser therapy (LLLT) can improve local tissue healing of bisphosphonate-induced osteonecrosis of the jaw. However, the effects of laser irradiation on bisphosphonate-treated osteoblasts have not been completely elucidated.

METHODS:

Human osteoblasts were cultured in plain culture medium (DMEM). After 48 h, plain DMEM was replaced by DMEM with no fetal bovine serum, for a 24-h incubation followed by addition of zoledronic acid (5 μM) for additional 48 h. Cells were subjected to LLLT (InGaAsP; 780 ± 3 nm; 0.025 W) at 0.5, 1.5, 3, 5, and 7 J/cm(2), three times every 24 h. Cell viability, total protein production, alkaline phosphatase activity (ALP), mineral nodule formation, gene expression of collagen type I and ALP, and cell morphology were evaluated.

RESULTS:

LLLT at 0.5 J/cm(2) increased cell viability of cultured osteoblasts. ALP activity and gene expression, in addition to mineral nodule formation and Col-I gene expression, were not increased by LLLT. LLLT applied to ZA-treated cells increased Col-I expression at 0.5, 1.5, and 3 J/cm(2) but did not improve any other cell activity assessed.

CONCLUSION:

LLLT showed limited effects on bisphosphonate-treated osteoblasts.

• Authors: Fernanda Gonçalves Basso, Ana Paula Silveira Turrioni, Diana Gabiela Soares, Vanderlei Salvador Bagnato, Josimeri Hebling, Carlos Alberto de Souza Costa
• Journal: Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer (Support Care Cancer) Vol. 22 Issue 10 Pg. 2741-8 (Oct 2014) ISSN: 1433-7339 [Electronic] Germany
• PMID: 24801347 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bone Density Conservation Agents
• Diphosphonates
• Imidazoles
• Zoledronic Acid

Topics

• Bisphosphonate-Associated Osteonecrosis of the Jaw (therapy)
• Bone Density Conservation Agents (pharmacology)
• Diphosphonates (pharmacology)
• Humans
• Imidazoles (pharmacology)
• Low-Level Light Therapy (methods)
• Osteoblasts (drug effects, physiology)
• Tumor Cells, Cultured
• Zoledronic Acid