[Immunomodulating activity of RNA mononucleotides].

Abstract

The immunological action of RNA mononucleotides was studied in animal experiments. The most pronounced activation of macrophagal glycolysis urea cycle, oxidative phosphorylation, lysosomal hydrolases was induced by uridine 5'-monophosphate (5'-UMP) and guanosine 5'-monophosphate (5'-GMP); 5'-GMP also induced the maximum increase of the expression of FC gamma receptors. 5'-UMP ensured cell activation comparable with the total action of all mononucleotides. 5'-UMP and 5'-GMP, used in combination, produced the highest stimulating effect on macrophages, and the addition of low-active adenosine 5'-monophosphate (5'-AMP) to active 5'-UMP did not decrease the stimulating potency of the latter. The stimulating activity of sodium nucleinate exceeded that of all mononucleotides and their combinations. 5'-GMP and 5'-AMP induced the maximum activation of oxygen metabolism, evaluated by chemiluminescence, while 5'-UMP and cytidine 5'-monophosphate (5'CMP) proved to be inactive. The shift of the phosphate group to the third carbon atom or the production of the oligonucleotide 5'-UMP consisting of 5-15 nucleotides resulted in the appearance of the capacity for stimulating oxygen metabolism in macrophages. Their in vitro cultivation with 5'-GMP and 5'-AMP induced the maximum increase of cell spreading in comparison with other mononucleotides, while the maximum increase of phagocytosis was ensured only by 5'-UMP and 5'-GMP.2+ 5'-UMP and 5'-GMP enhanced nonospecific resistance to Salmonella typhi infection, and sodium nucleinate, to Pseudomonas pseudomallei and Pseudomonas mallei infections.

• Authors: V M Zemskov, S V Rodionov, A V Khramtsov, A A Barsukov, V I Pantin
• Journal: Zhurnal mikrobiologii, epidemiologii i immunobiologii (Zh Mikrobiol Epidemiol Immunobiol) Issue 2 Pg. 58-63 (Feb 1988) ISSN: 0372-9311 [Print] Russia (Federation)
• Vernacular Title: Immunomoduliruiushchaia aktivnost' mononukleotidov RNK.
• PMID: 2452537 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Adjuvants, Immunologic
• Nucleotides
• Receptors, Fc
• Adenosine Monophosphate
• RNA
• Guanosine Monophosphate
• Uridine Monophosphate
• Cytidine Monophosphate

Topics

• Adenosine Monophosphate (pharmacology)
• Adjuvants, Immunologic
• Animals
• Cricetinae
• Cytidine Monophosphate (pharmacology)
• Dose-Response Relationship, Drug
• Guanosine Monophosphate (pharmacology)
• Immunization
• Luminescent Measurements
• Macrophages (drug effects, enzymology, immunology)
• Melioidosis (immunology)
• Mesocricetus
• Mice
• Mice, Inbred C57BL
• Mice, Inbred CBA
• Nucleotides (pharmacology)
• Phagocytosis (drug effects)
• RNA (pharmacology)
• Receptors, Fc (drug effects, immunology)
• Structure-Activity Relationship
• Typhoid Fever (immunology)
• Uridine Monophosphate (pharmacology)