Molecular classification of metaplastic carcinoma using surrogate immunohistochemical staining.

Abstract

OBJECTIVE:

The purpose of this study is to investigate molecular subtyping and its implications on metaplastic carcinoma according to surrogate immunohistochemical (IHC) staining.

METHODS:

Following tissue microarray analysis of 34 cases of metaplastic carcinoma, IHC staining for cytokeratin (CK) 5/6, epidermal growth factor receptor (EGFR), claudin-3, claudin-4, claudin-7, E-cadherin, STAT-1, androgen receptor and GGT was performed and classified into basal-like, molecular apocrine, claudin-low, immune-related, mixed and null types.

RESULTS:

Among the 34 cases of metaplastic carcinoma, 13 were of the basal-like type (35.2%), 9 of the mixed type (26.5%), 8 of the null type (23.5%), 3 of the claudin-low type (8.8%), and 1 was of the molecular apocrine type (2.9%). Depending on the cell type, there were differences between molecular subtypes, with the matrix-producing type occupying the largest proportion in the basal-like, null and mixed types. The spindle cell type represented the largest proportion in the claudin-low and molecular apocrine types, and the squamous cell type characterized the largest proportion in the basal-like type.

CONCLUSION:

Following molecular subtyping of metaplastic carcinomas using surrogate IHC markers, the largest number of cases was of the basal-like type, followed by the mixed, null, claudin-low and molecular apocrine types. There were differences between molecular subtypes according to the cell type.

• Authors: Hye Min Kim, Do Hee Kim, Woo Hee Jung, Ja Seung Koo
• Journal: Pathobiology : journal of immunopathology, molecular and cellular biology (Pathobiology) Vol. 81 Issue 2 Pg. 69-77 ( 2014) ISSN: 1423-0291 [Electronic] Switzerland
• PMID: 24356094 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 S. Karger AG, Basel.

Chemical References

• Biomarkers, Tumor

Topics

• Biomarkers, Tumor (analysis)
• Breast Neoplasms (classification, metabolism, pathology)
• Carcinoma (classification, metabolism, pathology)
• Female
• Humans
• Immunohistochemistry
• Middle Aged
• Tissue Array Analysis