Photothermal ablation of tumor cells using a single-walled carbon nanotube-peptide composite.

Abstract

Single-walled carbon nanotubes (SWCNTs) are known to have great potential for biomedical applications such as photothermal ablation of tumor cells in combination with near-infrared (NIR) irradiation. In this study, the photothermal activity of a novel SWCNTs composite with a designed peptide having a repeated structure of H-(-Lys-Phe-Lys-Ala-)7-OH [(KFKA)7] against tumor cells was evaluated in vitro and in vivo. The SWCNT-(KFKA)7 composite demonstrated high aqueous dispersibility that enabled SWCNTs to be used in tumor ablation. The NIR irradiation of SWCNT-(KFKA)7 solution resulted in a rapid temperature increase dependent on the SWCNTs concentration up to 50μg/ml. Three minutes of NIR irradiation of a colon 26 or HepG2 cell culture incubated with SWCNT-(KFKA)7 resulted in remarkable cell damage, while that by single treatment with SWCNT-(KFKA)7 or NIR irradiation alone was moderate. The intratumoral injection of SWCNT-(KFKA)7 solution followed by NIR irradiation resulted in a rapid increase of the temperature to 43°C in the subcutaneously inoculated colon 26 tumor based on thermographic observation and remarkable suppression of tumor growth compared with treatment with only SWCNT-(KFKA)7 injection alone or NIR irradiation alone. These results suggest the a great potential of an SWCNT-peptide composite for use in photothermal cancer therapy.

• Authors: Yasuhiko Hashida, Hironori Tanaka, Shuwen Zhou, Shigeru Kawakami, Fumiyoshi Yamashita, Tatsuya Murakami, Tomokazu Umeyama, Hiroshi Imahori, Mitsuru Hashida
• Journal: Journal of controlled release : official journal of the Controlled Release Society (J Control Release) Vol. 173 Pg. 59-66 (Jan 10 2014) ISSN: 1873-4995 [Electronic] Netherlands
• PMID: 24211651 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier B.V. All rights reserved.

Chemical References

• Antineoplastic Agents
• Nanotubes, Carbon
• Peptides

Topics

• Amino Acid Sequence
• Animals
• Antineoplastic Agents (chemistry, therapeutic use)
• Cell Line, Tumor
• Hep G2 Cells
• Humans
• Hyperthermia, Induced (methods)
• Mice
• Molecular Sequence Data
• Nanotubes, Carbon (chemistry)
• Neoplasms (pathology, therapy)
• Peptides (chemistry, therapeutic use)
• Phototherapy (methods)