MicroRNA-210 overexpression induces angiogenesis and neurogenesis in the normal adult mouse brain.
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| Abstract |
Angiogenesis and neurogenesis are crucial processes for brain tissue repair and remodeling after brain injury. Current study shows that microRNA-210 (miR-210) promotes vascular endothelial cell migration and tube formation under hypoxia in vitro. Whether miR-210 overexpression promotes focal angiogenesis and neurogenesis in the normal adult brain is unknown. Adult male C57BL/6 mice (n=54) underwent stereotactic injection of a lentiviral vector carrying miR-210 (LV-miR-210). Following 28 days of miR-210 gene transfer, endothelial cell and neural precursor cell proliferation, microvessel density and downstream angiogenic factor were genotyped. miR-210 was highly expressed in neurons, astrocytes and endothelial cells of the LV-miR-210-injected brain hemisphere. The endothelial cell proliferation and the number of newly formed microvessels were greatly increased in the LV-miR-210-treated mice compared with the controls (P<0.05). Neural progenitor cells in the subventricular zone were greatly increased compared with the controls (P<0.05). The data indicate that miR-210 is a key factor at the microRNA level in promoting angiogenesis and neurogenesis, which was associated with local increased vascular endothelial growth factor (VEGF) levels, suggesting that miR-210 may be a potential target for ischemic stroke therapy.
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| Authors | L Zeng, X He, Y Wang, Y Tang, C Zheng, H Cai, J Liu, Y Wang, Y Fu, G-Y Yang |
| Journal | Gene therapy (Gene Ther) Vol. 21 Issue 1 Pg. 37-43 (Jan 2014) ISSN: 1476-5462 [Electronic] England |
| PMID | 24152581 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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