To date, scientists have obtained a substantial amount of knowledge with regard to genes and
microRNAs (
miRNAs) in
pancreatic cancer (PC). However, deciphering the regulatory mechanism of these genes and
miRNAs remains difficult. In the present study, three regulatory networks consisting of a differentially-expressed network, a related network and a global network, were constructed in order to identify the mechanisms and certain key
miRNA and gene pathways in PC. The interactions between
transcription factors (TFs) and
miRNAs,
miRNAs and target genes and an
miRNA and its host gene were investigated. The present study compared and analyzed the similarities and differences between the three networks in order to distinguish the key pathways. Certain pathways involving the differentially-expressed genes and
miRNAs demonstrated specific features. TP53 and hsa-miR-125b were observed to form a self-adaptation association. A further 16 significant differentially-expressed
miRNAs were obtained and it was observed that an
miRNA and its host gene exhibit specific features in PC, for example, hsa-miR-196a-1 and its host gene, HOXB7, form a self-adaptation association. The differentially-expressed network partially illuminated the mechanism of PC. The present study provides comprehensive data that is associated with PC and may aid future studies in obtaining pertinent data results with regards to PC. In the future, an improved understanding of PC may be obtained through an increased knowledge of the occurrence, mechanism, improvement,
metastasis and treatment of the disease.