Multiparametric optical and MR imaging demonstrate inhibition of tumor angiogenesis natural history by mural cell therapy.

Abstract

PURPOSE:

To determine whether functional imaging using MRI and fibered confocal fluorescence microscopy (FCFM) could be used to monitor cell therapy by mural progenitor cells (MPC).

METHODS:

Fifty mice bearing TC1 murine xenograft tumors were allocated into: control (n = 17), sham (phosphate buffer saline, n = 16), and MPC-treated (MPC, n = 17) groups. MRI was performed before (D0 ) and 7 days (D7 ) after injection measuring tumor size, R2 * under air, oxygen, and carbogen using blood oxygen level dependent (BOLD) and f (fraction linked to microcirculation), D* (perfusion related coefficient) and Dr (restricted diffusion coefficient) using diffusion-weighted sequences based on the IVIM (intravoxel incoherent motion) method. FCFM was performed at D7 measuring "index leakage" (capillary permeability).

RESULTS:

Tumor growth was significantly slowed down in the MPC-treated animals (P = 0.002) on D7 . R2 *air significantly decreased in controls between D0 and D7 (P = 0.03), reflecting a decrease in tumor oxygenation. ΔR2 *O2CO2 significantly increased in controls between D0 and D7 (P = 0.01) reflecting loss of vessel response to carbogen. D* significantly decreased in controls between D0 and D7 (P = 0.03). Finally, "index leakage" was lower in the MPC-treated tumors (P = 0,009).

CONCLUSION:

Treatment by MPC resulted in slowing down of tumor growth, capillary permeability decrease, and stabilization of tumor angiogenesis.

• Authors: Nathalie Faye, Olivier Clément, Daniel Balvay, Victor Fitoussi, Laetitia Pidial, Federico Sandoval, Gwennhael Autret, Jean Sébastien Silvestre, Carole Leré Dean, Marianne Alison, Laure S Fournier
• Journal: Magnetic resonance in medicine (Magn Reson Med) Vol. 72 Issue 3 Pg. 841-9 (Sep 2014) ISSN: 1522-2594 [Electronic] United States
• PMID: 24123113 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 Wiley Periodicals, Inc.

Chemical References

• Ferric Compounds

Topics

• Animals
• Carcinoma, Squamous Cell (pathology)
• Cell- and Tissue-Based Therapy (methods)
• Cells, Cultured
• Diffusion Magnetic Resonance Imaging (methods)
• Ferric Compounds
• Heterografts
• Humans
• Mice
• Microcirculation
• Microscopy, Confocal (methods)
• Nanoparticles
• Staining and Labeling
• Stem Cell Transplantation (methods)
• Umbilical Cord (cytology)