The transcription analysis of duck enteritis virus UL49.5 gene using real-time quantitative reverse transcription PCR.

Abstract

Duck enteritis virus (DEV) UL49.5 encoding glycoprotein N was a conserved gene. The transcription dynamic process of UL49.5 homologous genes in herpesviruses was reported. However, the transcription dynamic process of DEV UL49.5 gene has not yet been established. In this study, a real-time quantitative reverse transcription PCR (real-time qRT-PCR) assay was established to test the transcription dynamic process of DEV UL49.5 gene, and the recombinant plasmid pUCm-T/UL49.5 was constructed as the standard DNA. The samples prepared from DEV-infected (at different time points) and uninfected cell were detected and calculated. The results demonstrated that the real-time qRT-PCR assay was successfully established. The transcription product of DEV UL49.5 gene was first detected at 0.5 h post infection (p.i.), increased at 8 h p.i. and reached a peak at 60 h p.i. Our results illustrated that DEV UL49.5 gene could be regarded as a late gene. The transcription dynamic process of DEV UL49.5 gene may provide a significant clue for further studies of DEV UL49.5 gene.

• Authors: Meng Lin, Renyong Jia, Mingshu Wang, Xinghong Gao, Dekang Zhu, Shun Chen, Zhongqiong Yin, Yin Wang, Xiaoyue Chen, Anchun Cheng
• Journal: Virus genes (Virus Genes) Vol. 47 Issue 2 Pg. 298-304 (Oct 2013) ISSN: 1572-994X [Electronic] United States
• PMID: 23836488 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glycoproteins
• Viral Structural Proteins

Topics

• Animals
• Cells, Cultured
• Ducks
• Fibroblasts (virology)
• Gene Expression Profiling
• Glycoproteins (biosynthesis, genetics)
• Mardivirus (genetics, growth & development)
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction
• Time Factors
• Transcription, Genetic
• Viral Structural Proteins (biosynthesis, genetics)