ESAT-6-gpi DNA vaccine augmented the specific antitumour efficacy induced by the tumour vaccine B16F10-ESAT-6-gpi/IL-21 in a mouse model.

Abstract

In this study, we hypothesized that the mice immunized with the glycosylphosphatidylinositol (GPI) anchored 6-kDa early-secreted antigenic target (ESAT-6) DNA vaccine (ESAT-6-gpi) and the tumour vaccine B16F10-ESAT-6-gpi/IL-21 might significantly enhance immune responses and antimelanoma efficacy. Our experimental results indicated that the anti-ESAT-6 antibody induced by the DNA vaccine ESAT-6-gpi bound ESAT-6 to the surface of tumour vaccine to activate a complement classical pathway and resulted in the B16F10 tumour cell lysis and apoptosis, which served as a potential trigger for breaking melanomatous immune tolerance to elicit an initiation of natural antimelanoma immunity. Our innovative approach of using the DNA vaccine ESAT-6-gpi priming and the tumour vaccine B16F10-ESAT-6-gpi/IL-21 boosting induced strong antimelanoma immunity that inhibited melanomatous growth. These findings highlighted the DNA vaccine ESAT-6-gpi as an immune enhancer to augment the immune efficacy of the tumour vaccine B16F10-ESAT -6-gpi/IL-21 against melanoma in a mouse model.

• Authors: X He, J Wang, F Zhao, D Chen, J Chen, H Zhang, C Yang, Y Liu, J Dou
• Journal: Scandinavian journal of immunology (Scand J Immunol) Vol. 78 Issue 1 Pg. 69-78 (Jul 2013) ISSN: 1365-3083 [Electronic] England
• PMID: 23679337 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 John Wiley & Sons Ltd.

Chemical References

• Antigens, Bacterial
• Bacterial Proteins
• Cancer Vaccines
• ESAT-6 protein, Mycobacterium tuberculosis
• Glycosylphosphatidylinositols
• Interleukins
• Vaccines, DNA
• plasmanylinositol glycan
• interleukin-21

Topics

• Animals
• Antigens, Bacterial (immunology)
• Bacterial Proteins (immunology)
• Cancer Vaccines (immunology)
• Cell Line, Tumor
• Glycosylphosphatidylinositols (administration & dosage)
• Immunization
• Interleukins (immunology)
• Melanoma, Experimental (therapy)
• Mice
• Mice, Inbred C57BL
• Vaccines, DNA (immunology)