Abstract | AIMS: METHODS: Human cholangiocarcinoma QBC939 stable cell lines with over-expressed or silenced NGF-β genes were generated with pEGFP-N1-NGF-β and pGPU6/GFP/Neo- NGF-β- shRNA recombinant plasmids. Cell proliferation assay, colony formation assay, cell cycle analysis, apoptosis assay and tumorigenicity assay were performed to evaluate the role of NGF-β in the progression of human cholangiocarcinoma. In addition, human lymphatic endothelial cells were co-cultured with QBC939 culture supernatants, and the cell proliferation and migration abilities of the lymphatic endothelial cells were evaluated. RESULTS: Forced expression of NGF-β in QBC939 cell lines promoted proliferation, colony formation and tumorigenicity in these cells and inhibited the apoptosis. However, down-regulation of NGF-β inhibited proliferation, colony formation and tumorigenicity, and increased the apoptotic rate of QBC939 cells. In addition, the NGF-β gain-of-function induced a high expression of vascular endothelial growth factor C and enhanced the proliferation and migration of lymphatic endothelial cells, while NGF-β loss-of-function showed opposite effects. CONCLUSIONS:
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Authors | Xiu-Jing Yue, Lei-Bo Xu, Man-Sheng Zhu, Rui Zhang, Chao Liu |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 4
Pg. e62024
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 23637956
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Vascular Endothelial Growth Factor C
- Nerve Growth Factor
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Topics |
- Animals
- Apoptosis
(genetics)
- Cell Cycle
(genetics)
- Cell Line, Tumor
- Cell Movement
(genetics)
- Cell Proliferation
- Cell Transformation, Neoplastic
(genetics)
- Cholangiocarcinoma
(genetics, pathology)
- Disease Progression
- Female
- Gene Expression
- Gene Expression Regulation, Neoplastic
- Gene Order
- Gene Silencing
- Heterografts
- Humans
- Mice
- Nerve Growth Factor
(genetics)
- Tumor Burden
(genetics)
- Vascular Endothelial Growth Factor C
(genetics)
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