Treatment of neural anosmia by topical application of basic fibroblast growth factor-gelatin hydrogel in the nasal cavity: an experimental study in mice.

Abstract

IMPORTANCE:

A new treatment of neural anosmia.

OBJECTIVE:

To investigate the effects of basic fibroblast growth factor (bFGF)-gelatin hydrogel on recovery of neural anosmia in mice.

DESIGN:

Anosmia was induced by intraperitoneal injection of 3-methylindole, 200 mg/kg. One week later, the animals underwent 1 of the following 3 procedures bilaterally: (1) group A: single-shot intranasal drip infusion of phosphate-buffered saline, (2) group B: single-shot intranasal drip infusion of bFGF, and (3) group C: placement of bFGF-gelatin hydrogel in the nasal cavity. The olfactory function of the animal was evaluated by the odor-detection test (ODT) 2 and 4 weeks later. Following the testing, the animal was killed, the thickness of the olfactory epithelium was measured, and the number of olfactory marker protein (OMP)-positive cells was counted.

SETTING:

Research installation.

PARTICIPANTS:

Mice.

INTERVENTION:

The placement of bFGF-gelatin hydrogel in the nasal cavity.

MAIN OUTCOMES AND MEASURES:

An ODT, thickness of olfactory epithelium, the number of OMP-positive cells

RESULTS:

The ODT proved that neural anosmia recovered in group C but not in groups A and B. Histologically, olfactory epithelium became thicker and the number of OMP-positive cells increased in group C, while such functional and histologic recovery was poor in groups A and B. These findings suggested that placement of bFGF-gelatin hydrogel in the nasal cavity was an efficient way to facilitate recovery of neural anosmia.

CONCLUSIONS AND RELEVANCE:

As a gelatin hydrogel degrades slowly in the body, bFGF is gradually released around the site of the lesion; thus, it constantly exerts its effects on neural regeneration.

• Authors: Jumpei Nota, Hirotaka Takahashi, Nobuhiro Hakuba, Naohito Hato, Kiyofumi Gyo
• Journal: JAMA otolaryngology-- head & neck surgery (JAMA Otolaryngol Head Neck Surg) Vol. 139 Issue 4 Pg. 396-400 (Apr 2013) ISSN: 2168-619X [Electronic] United States
• PMID: 23599076 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fibroblast Growth Factor 2
• Hydrogel, Polyethylene Glycol Dimethacrylate
• Gelatin

Topics

• Administration, Topical
• Animals
• Disease Models, Animal
• Female
• Fibroblast Growth Factor 2 (administration & dosage, therapeutic use)
• Gelatin (administration & dosage)
• Hydrogel, Polyethylene Glycol Dimethacrylate (administration & dosage)
• Mice
• Mice, Inbred BALB C
• Nasal Cavity
• Olfaction Disorders (drug therapy, physiopathology)
• Olfactory Mucosa (drug effects)
• Olfactory Nerve (drug effects, physiopathology)
• Smell (physiology)
• Treatment Outcome