Radixin expression in microglia after cortical stroke lesion.

Abstract

Stroke induces extensive tissue remodeling, resulting in the activation of several cell types in the brain as well as recruitment of blood-borne leucocytes. Radixin is part of a cytoskeleton linker protein family with the ability to connect transmembrane proteins to the actin cytoskeleton, promoting cell functions involving a dynamic cytoskeleton such as morphological changes, cell division and migration which are common events of different cell types after stroke. In the healthy adult brain radixin is expressed in Olig2(+) cells throughout the brain and in neural progenitor cells in the subventricular zone. In the current study, we detected a 2.5 fold increase in the number of radixin positive cells in the peri-infarct cortex two weeks after the induction of cortical stroke by photothrombosis. Similarly, the number of Olig2(+) cells increased in the peri-infarct area after stroke; however, the number of radixin(+)/Olig2(+) cells was unchanged. Neural progenitor cells maintained radixin expression on their route to the infarct. More surprising however, was the expression of radixin in activated microglia in the peri-infarct cortex. Seventy percent of Iba1(+) cells expressed radixin after stroke, a population which was not present in the control brain. Furthermore, activation of radixin was predominantly detected in the peri-infarct region of oligodendrocyte progenitors and microglia. The specific location of radixin(+) cells in the peri-infarct region and in microglia suggests a role for radixin in microglial activation after stroke.

• Authors: Åsa Persson, Ahmed Osman, Hayde Bolouri, Carina Mallard, H Georg Kuhn
• Journal: Glia (Glia) Vol. 61 Issue 5 Pg. 790-9 (May 2013) ISSN: 1098-1136 [Electronic] United States
• PMID: 23440885 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Wiley Periodicals, Inc.

Chemical References

• Cytoskeletal Proteins
• Membrane Proteins
• radixin

Topics

• Animals
• Animals, Newborn
• Cells, Cultured
• Cerebral Cortex (metabolism, pathology)
• Cytoskeletal Proteins (biosynthesis, genetics)
• Female
• Membrane Proteins (biosynthesis, genetics)
• Mice
• Mice, Inbred C57BL
• Microglia (metabolism, pathology)
• Stroke (metabolism, pathology)