Abstract |
The killing effect of nitrogen-doped titanium dioxide (N-TiO2) nanoparticles on human cervical carcinoma (HeLa) cells by visible light photodynamic therapy ( PDT) was higher than that of TiO2 nanoparticles. To study the mechanism of the killing effect, the reactive oxygen species produced by the visible-light-activated N-TiO2 and pure-TiO2 were evaluated and compared. The changes of the cellular parameters, such as the mitochondrial membrane potential ( MMP), intracellular Ca2+, and nitrogen monoxide (NO) concentrations after PDT were measured and compared for N-TiO2- and TiO2-treated HeLa cells. The N-TiO2 resulted in more loss of MMP and higher increase of Ca2+ and NO in HeLa cells than pure TiO2. The cell morphology changes with time were also examined by a confocal microscope. The cells incubated with N-TiO2 exhibited serious distortion and membrane breakage at 60 min after the PDT.
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Authors | Zheng Li, Xiaobo Pan, Tianlong Wang, Pei-Nan Wang, Ji-Yao Chen, Lan Mi |
Journal | Nanoscale research letters
(Nanoscale Res Lett)
Vol. 8
Issue 1
Pg. 96
(Feb 22 2013)
ISSN: 1931-7573 [Print] United States |
PMID | 23433090
(Publication Type: Journal Article)
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