Lysophosphatidic acid enhances collagen deposition and matrix thickening in engineered tissue.

Abstract

The time needed to produce engineered tissue is critical. A self-assembly approach provided excellent results regarding biological functions and cell differentiation because it closely respected the microenvironment of cells. Nevertheless, the technique was time consuming for producing tissue equivalents with enough extracellular matrix to allow manipulations. Unlike L-arginine supplementation that only increased accumulation of collagen in cell culture supernatant in our model, addition of lysophosphatidic acid, a natural bioactive lipid, did not modify the amount of accumulated collagen in the cell culture supernatant; however, it enhanced the matrix deposition rate without inducing fibroblast hyperproliferation and tissue fibrosis.

• Authors: Stéphane Chabaud, Thomas-Louis Marcoux, Marie-Pier Deschênes-Rompré, Alexandre Rousseau, Amélie Morissette, Sara Bouhout, Geneviève Bernard, Stéphane Bolduc
• Journal: Journal of tissue engineering and regenerative medicine (J Tissue Eng Regen Med) Vol. 9 Issue 11 Pg. E65-75 (Nov 2015) ISSN: 1932-7005 [Electronic] England
• PMID: 23418181 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 John Wiley & Sons, Ltd.

Chemical References

• Culture Media
• Fibronectins
• Lipids
• Lysophospholipids
• Collagen
• Arginine
• lysophosphatidic acid

Topics

• Arginine (chemistry)
• Biopsy
• Cadaver
• Cell Differentiation
• Cell Proliferation
• Collagen (chemistry)
• Culture Media (chemistry)
• Epithelial Cells (cytology)
• Extracellular Matrix (metabolism)
• Fibroblasts (cytology, metabolism)
• Fibronectins (chemistry)
• Humans
• Lipids (chemistry)
• Lysophospholipids (chemistry)
• Microscopy, Fluorescence
• Phenotype
• Skin (metabolism, pathology)
• Tissue Engineering (methods)
• Urothelium (metabolism)