[Expression of HIF-1a in acute limb ischemia of rats].

Abstract

OBJECTIVE:

To explore the expression of hypoxia-inducible factor 1 alpha (HIF-1a) in rat lower limb skeletal muscle with acute ischemia.

METHODS:

Acute lower limb ischemia was established in 25 male or female SD rats of 200-250 g by ligating left iliac artery with microsurgical techniques. Gastrocnemius of lower limb was harvest at the time points of sham (0h), 4, 8, 12 and 24 h respectively. Immunohistochemistry, Western blot and real-time polymerase chain reaction (PCR) were used to detect the expression of HIF-1a in lower limb gastrocnemius.

RESULTS:

There was a low expression of HIF-1a in normal gastrocnemius, but it was highly expressed in lower limb ischemia. With the elongation of ischemia time, its expression increased (P < 0.05), peaked at 4 h and 8 h (P < 0.05) and then declined gradually.

CONCLUSION:

With a characteristic trend during acute limb ischemia, HIF-1a may play an important role in the adaptive response to acute limb ischemia.

• Authors: Xi Li, Shi-jie Xin, Dong Yang, Kui Ding, Ying-hou Wang, Hui Cao, Jian Zhang, Zhi-quan Duan
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 92 Issue 45 Pg. 3232-5 (Dec 04 2012) ISSN: 0376-2491 [Print] China
• PMID: 23328475 (Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Hif1a protein, rat
• Hypoxia-Inducible Factor 1, alpha Subunit

Topics

• Animals
• Female
• Hypoxia-Inducible Factor 1, alpha Subunit (metabolism)
• Ischemia (metabolism)
• Lower Extremity (blood supply)
• Male
• Muscle, Skeletal (metabolism)
• Rats
• Rats, Sprague-Dawley