Dietary modulation of plasma angiopoietin-like protein 4 concentrations in healthy volunteers and in patients with type 2 diabetes.

Abstract

BACKGROUND:

Angiopoietin-like protein 4 (ANGPTL4) has been identified as an inhibitor of lipoprotein lipase. Preliminary data suggest that plasma nonesterified fatty acids (NEFAs) raise plasma ANGPTL4 concentrations in humans.

OBJECTIVE:

The objective was to assess plasma ANGPTL4 concentrations after various nutritional interventions that increase NEFA concentrations in healthy subjects and in patients with type 2 diabetes mellitus.

DESIGN:

We studied 4 groups, both at baseline and after 3 d of either fasting (n = 22 healthy men), a very-low-calorie diet (VLCD; n = 10 healthy men and n = 10 patients with diabetes), or a high-fat, high-energy diet (HFED; n = 15 healthy men). Plasma ANGPTL4, NEFA, and triglyceride concentrations were measured.

RESULTS:

In healthy men, a VLCD increased ANGPTL4 from 13.2 (IQR: 8.1-24.2) at baseline to 18.2 (16.7-33.4) ng/mL (P < 0.05), fasting increased ANGPTL4 from 10.6 (7.6-17.6) to 28.0 (23.1-35.0) ng/mL (P < 0.05), and an HFED increased ANGPTL4 from 13.9 (8.2-22.0) to 17.2 (11.2-23.6) ng/mL (P < 0.05). In men with diabetes, a VLCD also increased ANGPTL4, from 10.9 ± 2.4 to 19.2 ± 3.2 ng/mL (P < 0.05). All interventions significantly increased plasma NEFAs in both healthy men and patients with diabetes. The change in ANGPTL4 positively correlated with the change in NEFA concentrations (β = 0.048, P < 0.001) and negatively correlated with the change in plasma triglycerides (β = -0.051, P = 0.01).

CONCLUSIONS:

Three days of either fasting, a VLCD, or an HFED increased plasma ANGPTL4 concentrations in healthy men, concomitantly with increased plasma NEFA concentrations. Similarly, a VLCD in patients with diabetes increased ANGPTL4 concentrations, concomitantly with increased NEFA concentrations.

• Authors: Jacqueline T Jonker, Johannes W A Smit, Sebastiaan Hammer, Marieke Snel, Rutger W van der Meer, Hildo J Lamb, Frits Mattijssen, Karin Mudde, Ingrid M Jazet, Olaf M Dekkers, Albert de Roos, Johannes A Romijn, Sander Kersten, Patrick C N Rensen
• Journal: The American journal of clinical nutrition (Am J Clin Nutr) Vol. 97 Issue 2 Pg. 255-60 (Feb 2013) ISSN: 1938-3207 [Electronic] United States
• PMID: 23283507 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ANGPTL4 protein, human
• Angiopoietin-Like Protein 4
• Angiopoietins
• Fatty Acids, Nonesterified
• Triglycerides

Topics

• Adult
• Angiopoietin-Like Protein 4
• Angiopoietins (blood)
• Caloric Restriction
• Diabetes Mellitus, Type 2 (blood, diet therapy)
• Diet, High-Fat (adverse effects)
• Energy Intake
• Enzyme-Linked Immunosorbent Assay
• Fasting (adverse effects)
• Fatty Acids, Nonesterified (blood)
• Humans
• Male
• Middle Aged
• Triglycerides (blood)
• Up-Regulation
• Young Adult