Abstract |
Previous study showed that aberrant HLA-G expression in cancer cells plays important roles in disease progression and it was associated with tumor metastasis and with poor survival in an animal model with ovarian cancer; however, the mechanisms remain to be explored. In this study, we found that HLA-G expression could dramatically decreased the NK cytotoxicity against the ovarian carcinoma cell line (HO-8910) engineered to express HLA-G (HO-8910-G), and matrix metalloproteinase-15 (MMP-15) expression was up-regulated in HO-8910-G cells. Consistent with this, a strong correlation between HLA-G and MMP-15 expression were observed in a cohort of ovarian cancer samples. Knockdown the HLA-G induced MMP-15 expression by small interfere RNA ( siRNA) significantly decreased the HO-8910-G cell migration potential and tumor metastasis. Collectively, our study indicated that HLA-G involved in tumor invasiveness or metastasis may rely on the NK cytotoxicity inhibition and induction of MMP-15 expression in ovarian cancer.
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Authors | Aifen Lin, Hui-Hui Xu, Dan-Ping Xu, Xia Zhang, Qing Wang, Wei-Hua Yan |
Journal | Human immunology
(Hum Immunol)
Vol. 74
Issue 4
Pg. 439-46
(Apr 2013)
ISSN: 1879-1166 [Electronic] United States |
PMID | 23228395
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- HLA-G Antigens
- RNA, Small Interfering
- Matrix Metalloproteinase 15
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Topics |
- Animals
- Carcinoma
(genetics, immunology, pathology)
- Cell Line, Tumor
- Cell Movement
- Cytotoxicity, Immunologic
- Female
- Gene Expression Regulation, Neoplastic
(immunology)
- HLA-G Antigens
(genetics, immunology)
- Humans
- Killer Cells, Natural
(immunology, pathology)
- Matrix Metalloproteinase 15
(genetics, immunology)
- Mice
- Mice, Nude
- Neoplasm Metastasis
- Neoplasm Transplantation
- Ovarian Neoplasms
(genetics, immunology, pathology)
- RNA, Small Interfering
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