Canonical and atypical E2Fs regulate the mammalian endocycle.

Abstract

The endocycle is a variant cell cycle consisting of successive DNA synthesis and gap phases that yield highly polyploid cells. Although essential for metazoan development, relatively little is known about its control or physiologic role in mammals. Using lineage-specific cre mice we identified two opposing arms of the E2F program, one driven by canonical transcription activation (E2F1, E2F2 and E2F3) and the other by atypical repression (E2F7 and E2F8), that converge on the regulation of endocycles in vivo. Ablation of canonical activators in the two endocycling tissues of mammals, trophoblast giant cells in the placenta and hepatocytes in the liver, augmented genome ploidy, whereas ablation of atypical repressors diminished ploidy. These two antagonistic arms coordinate the expression of a unique G2/M transcriptional program that is critical for mitosis, karyokinesis and cytokinesis. These results provide in vivo evidence for a direct role of E2F family members in regulating non-traditional cell cycles in mammals.

• Authors: Hui-Zi Chen, Madhu M Ouseph, Jing Li, Thierry Pécot, Veda Chokshi, Lindsey Kent, Sooin Bae, Morgan Byrne, Camille Duran, Grant Comstock, Prashant Trikha, Markus Mair, Shantibhusan Senapati, Chelsea K Martin, Sagar Gandhi, Nicholas Wilson, Bin Liu, Yi-Wen Huang, John C Thompson, Sundaresan Raman, Shantanu Singh, Marcelo Leone, Raghu Machiraju, Kun Huang, Xiaokui Mo, Soledad Fernandez, Ilona Kalaszczynska, Debra J Wolgemuth, Piotr Sicinski, Tim Huang, Victor Jin, Gustavo Leone
• Journal: Nature cell biology (Nat Cell Biol) Vol. 14 Issue 11 Pg. 1192-202 (Nov 2012) ISSN: 1476-4679 [Electronic] England
• PMID: 23064266 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• E2F Transcription Factors
• E2F1 Transcription Factor
• E2F2 Transcription Factor
• E2F3 Transcription Factor
• E2F7 Transcription Factor
• E2F8 protein, mouse
• Repressor Proteins

Topics

• Animals
• Cell Cycle (genetics, physiology)
• Chromatin Immunoprecipitation
• E2F Transcription Factors (genetics, metabolism)
• E2F1 Transcription Factor (genetics, metabolism)
• E2F2 Transcription Factor (genetics, metabolism)
• E2F3 Transcription Factor (genetics, metabolism)
• E2F7 Transcription Factor (genetics, metabolism)
• Female
• Flow Cytometry
• Giant Cells (cytology, metabolism)
• Hepatocytes (cytology, metabolism)
• Immunohistochemistry
• Mice
• Microscopy, Confocal
• Microscopy, Electron, Transmission
• Microscopy, Fluorescence
• Pregnancy
• Repressor Proteins (genetics, metabolism)
• Trophoblasts (metabolism)