Decreased miR-7 expression in the skin and sera of patients with dermatomyositis.

Abstract

Expression of microRNA (miRNA) in the skin in dermatomyositis has not previously been studied in detail. In this study, we performed miRNA array analysis using miRNAs purified from dermatomyositis-involved skin and normal skin, and found that several miRNAs were up- or down-regulated in dermatomyositis skin. Among them, we focused on miR-7, one of the most down-regulated miRNAs in dermatomyositis skin. Total miRNAs were purified from serum, and hsa-miR-7 levels were measured with quantitative real-time PCR using the specific primer. Serum levels of miR-7 were significantly decreased in patients with dermatomyositis compared with normal subjects or patients with other autoimmune diseases. Thus, serum miR-7 levels might be a possible diagnostic marker for dermatomyositis. Clarifying the up- or down-stream events of down-regulated miR-7 in patients with dermatomyositis may lead to further understanding of the disease and a new therapeutic approach.

• Authors: Yuka Oshikawa, Masatoshi Jinnin, Takamitsu Makino, Ikko Kajihara, Katsunari Makino, Noritoshi Honda, Wakana Nakayama, Kuniko Inoue, Satoshi Fukushima, Hironobu Ihn
• Journal: Acta dermato-venereologica (Acta Derm Venereol) Vol. 93 Issue 3 Pg. 273-6 (May 2013) ISSN: 1651-2057 [Electronic] Sweden
• PMID: 23007163 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Genetic Markers
• MIRN7 microRNA, human
• MicroRNAs

Topics

• Case-Control Studies
• Dermatomyositis (blood, genetics)
• Down-Regulation
• Gene Expression Profiling (methods)
• Genetic Markers
• Humans
• MicroRNAs (analysis, blood)
• Oligonucleotide Array Sequence Analysis
• Prognosis
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction
• Skin (chemistry)