Abstract | PURPOSE: Expression of the adhesion molecule L1-CAM (L1) has been shown to correlate with early recurrence in breast cancer. Here, we investigated whether L1-CAM expression of breast cancer cells might influence adherence to human pulmonary microvascular endothelial cells (HPMEC) and thus promote metastasis. METHODS: MDA-MB231-Fra2 breast cancer cells that express high levels of L1-CAM (L1(high) cells) were stably transfected to generate clones with strong L1-CAM downregulation. Adhesion to activated HPMEC was studied in dynamic cell flow and static assays. Potential binding partners on endothelial cells were identified by blocking experiments and adhesion assays after coating of the flow channels with recombinant proteins. RESULTS: CONCLUSIONS: Our experiments indicate that L1-CAM expression on breast cancer cells can promote adherence to activated endothelial cells by binding to endothelial L1-CAM or ALCAM. This mechanism might lead to increased metastasis and a poor prognosis in L1-CAM-positive carcinomas in vivo. Therefore, L1-CAM might be a suitable therapeutic target in breast cancers with a high L1-CAM expression.
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Authors | Virginia Dippel, Karin Milde-Langosch, Daniel Wicklein, Udo Schumacher, Peter Altevogt, Leticia Oliveira-Ferrer, Fritz Jänicke, Christine Schröder |
Journal | Journal of cancer research and clinical oncology
(J Cancer Res Clin Oncol)
Vol. 139
Issue 1
Pg. 107-21
(Jan 2013)
ISSN: 1432-1335 [Electronic] Germany |
PMID | 22983139
(Publication Type: Journal Article)
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Chemical References |
- ALCAM protein, human
- Antigens, CD
- Cell Adhesion Molecules, Neuronal
- E-Selectin
- Fetal Proteins
- Neural Cell Adhesion Molecule L1
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Topics |
- Antigens, CD
(metabolism)
- Blotting, Western
- Breast Neoplasms
(metabolism, pathology)
- Cell Adhesion
- Cell Adhesion Molecules, Neuronal
(metabolism)
- Cell Line, Tumor
- E-Selectin
(metabolism)
- Endothelial Cells
(metabolism)
- Female
- Fetal Proteins
(metabolism)
- Flow Cytometry
- Gene Expression Regulation, Neoplastic
- Humans
- Neural Cell Adhesion Molecule L1
(metabolism)
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