Establishment of reproducible xenotransplantation model of T cell acute lymphoblastic leukemia in NOD/SCID mice.

Abstract

T cell acute lymphoblastic leukemia (T-ALL) is an aggressive leukemia. However the poor prognosis and low morbidity restrict further analysis of the disease. Therefore there is an increasing demand to develop animal models for identifying novel therapeutic approaches. In this study, we inoculated the anti-mouse CD122 monoclonal antibody conditioned NOD/SCID mice with the leukemia cells from 9 T-ALL patients and 1 cell line via the tail vein. Four of the 9 patients and the cell line were successfully engrafted. Flow cytometry detected high percentage of human CD45(+) cells in recipient mice. Immunohistochemistry showed infiltration of human CD45(+) cells in different organs. Serial transplantation was also achieved. In vivo drug treatment showed that dexamethasone could extend survival, which was consistent with clinical observation. These results demonstrated that we successfully established 5 xenotransplantation models of T-ALL in anti-mCD122 mAb conditioned NOD/SCID mice, which recapitulated the characteristics of original disease.

• Authors: Di Wang, Na Wang, Yan Zhang, Shuyan Ma, Zhe Geng, Pengfei Zhou, Jianfeng Zhou, Liang Huang
• Journal: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (J Huazhong Univ Sci Technolog Med Sci) Vol. 32 Issue 4 Pg. 511-516 (Aug 2012) ISSN: 1672-0733 [Print] China
• PMID: 22886962 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Disease Models, Animal
• Mice
• Mice, Inbred NOD
• Mice, SCID
• Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
• Transplantation, Heterologous (methods)