Up-regulation of thy-1 promotes invasion and metastasis of hepatocarcinomas.

Abstract

Increasing evidence has revealed that thy-1 was a potential stem cell marker of liver cancer, but no data have been shown on how thy-1 regulates the pathophysiology of liver cancer, such as proliferation, apoptosis, invasion and migration. We previously demonstrated that thy-1 was expressed in about 1% of hepg2 cells, thy-1+ hepg2 cells, but not thy-1-, demonstrating high tumorigenesis on inoculation 0.5x10⁵ cells per BACA/LA mouse after 2 months. In the present study, our results showed that higher expression of thy-1 occurs in 72% (36/50 cases) of neoplastic hepatic tissues as compared to 40% (20/50 cases) of control tissues, and the expression of thy-1 is higher in poorly differentiated liver tumors than in the well-differentiated ones. In addition, thy-1 expression was detected in 85% of blood samples from liver cancer patients, but none in normal subjects or patients with cirrhosis or hepatitis. There was a significant negative correlation between thy-1 expression and E-cadherin expression (a marker of invasion and migraton), but not between thy-1 expression and AFP expression in all the liver cancer and blood samples. We further investigated the relationship between thy-1 and E- cadherin in liver cancer hepg2 cell line which was transfected with pReceiver-M29/thy-1 eukaryotic expression vector followed by aspirin treatment. Lower expression of E- cadherin but higher expressions of thy-1 were detected in hepg2 cells transfected with pReceiver-M29/thy-1. Taken together, our study suggested that thy-1 probably regulates liver cancer invasion and migration.

• Authors: Bian-Qiao Cheng, Yi Jiang, Dong-Liang Li, Jing-Jing Fan, Ming Ma
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 13 Issue 4 Pg. 1349-53 ( 2012) ISSN: 2476-762X [Electronic] Thailand
• PMID: 22799330 (Publication Type: Journal Article)

Chemical References

• Cadherins
• Thy-1 Antigens
• alpha-Fetoproteins
• Aspirin

Topics

• Analysis of Variance
• Aspirin (pharmacology)
• Cadherins (drug effects, metabolism)
• Carcinoma (blood, metabolism, pathology)
• Cell Differentiation
• Chi-Square Distribution
• Hep G2 Cells
• Humans
• Liver (metabolism)
• Liver Neoplasms (blood, metabolism, pathology)
• Thy-1 Antigens (blood, drug effects, metabolism)
• Transfection
• Up-Regulation
• alpha-Fetoproteins (drug effects, metabolism)