Highly efficient cationic ethylphosphatidylcholine siRNA carrier for GFP suppression in modified breast cancer cells.

Abstract

AIM:

Cationic ethylphosphatidylcholines (ePCs) were evaluated for the delivery of siRNA in modified breast cancer cells.

MATERIALS AND METHODS:

Dimyristoleoyl-ePC (C14), dioleoyl-ePC (C18), and dilauroyl-ePC (C12) nanoparticles were complexed with siRNA for green fluorescent protein (GFP) suppression in modified MCF-7 breast cancer cells. The kinetics of GFP suppression were followed over the course of 72 hours.

RESULTS:

C14, which has been previously found to be particularly effective in gene transfection into primary human umbilical artery endothelial cells, was also remarkably effective as siRNA carrier, with an efficacy exceeding that of Lipofectamine RNAiMAX. The C14 toxicity remained comparable to that of RNAiMAX. The efficacy of the other tested cationic ePC formulations was less than that of C14 and RNAiMAX.

CONCLUSION:

The cationic lipid C14 is a highly efficient siRNA carrier that could be used for the development of new formulations for siRNA delivery into cancer cells. A valuable advantage of the C14 formulations is the fact that they are simple, and do not require adjuvants or complex preparation procedures.

• Authors: Boris Tenchov, Yasuro Sugimoto, Rumiana Koynova, Robert W Brueggemeier, Robert J Lee
• Journal: Anticancer research (Anticancer Res) Vol. 32 Issue 7 Pg. 2563-6 (Jul 2012) ISSN: 1791-7530 [Electronic] Greece
• PMID: 22753714 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Cations
• Oleic Acids
• Phosphatidylcholines
• RNA, Small Interfering
• o-ethyldioleoylphosphatidylcholinium
• Green Fluorescent Proteins

Topics

• Breast Neoplasms (genetics, therapy)
• Cations (administration & dosage, chemistry)
• Cell Line, Tumor
• Female
• Genetic Therapy (methods)
• Genetic Vectors (chemistry, genetics)
• Green Fluorescent Proteins
• Humans
• Nanoparticles (administration & dosage, chemistry)
• Oleic Acids (administration & dosage, chemistry)
• Phosphatidylcholines (administration & dosage, chemistry)
• RNA, Small Interfering (administration & dosage, chemistry)
• Structure-Activity Relationship
• Transfection (methods)