Abstract |
This study quantified the effects of local intramedullary delivery of an organic vanadium salt, which may act as an insulin-mimetic on fracture healing. Using a BB Wistar rat femoral fracture model, local vanadyl acetylacetonate (VAC) was delivered to the fracture site and histomorphometry, mechanical testing, and immunohistochemistry were performed. Callus percent cartilage was 200% higher at day 7 (p < 0.05) and 88% higher at day 10 (p < 0.05) in the animals treated with 1.5 mg/kg of VAC. Callus percent mineralized tissue was 37% higher at day 14 (p < 0.05) and 31% higher at day 21 (p < 0.05) in the animals treated with 1.5 mg/kg of VAC. Maximum torque to failure was 104% and 154% higher at 4 weeks post-fracture (p < 0.05) for the healing femurs from the VAC-treated (1.5 and 3.0 mg/kg) animals. Animals treated with other VAC doses demonstrated increased mechanical parameters at 4 weeks (p < 0.05). Immunohistochemistry detected 62% more proliferating cells at days 7 (p < 0.05) and 94% more at day 10 (p < 0.05) in the animals treated with 1.5 mg/kg VAC. Results showed 100% more vascular endothelial growth factor-C ( VEGF-C) positive cells and 80% more blood vessels at day 7 (p < 0.05) within the callus subperiosteal region of VAC-treated animals (1.5 mg/kg) compared to controls. The results suggest that local VAC treatment affects chondrogenesis and angiogenesis within the first 7-10 days post-fracture, which leads to enhanced mineralized tissue formation and accelerated fracture repair as early as 3-4 weeks post-fracture.
|
Authors | David N Paglia, Aaron Wey, Andrew G Park, Eric A Breitbart, Siddhant K Mehta, John D Bogden, Francis W Kemp, Joseph Benevenia, J Patrick O'Connor, Sheldon S Lin |
Journal | Journal of orthopaedic research : official publication of the Orthopaedic Research Society
(J Orthop Res)
Vol. 30
Issue 12
Pg. 1971-8
(Dec 2012)
ISSN: 1554-527X [Electronic] United States |
PMID | 22653614
(Publication Type: Journal Article)
|
Copyright | Copyright © 2012 Orthopaedic Research Society. |
Chemical References |
- Hydroxybutyrates
- Insulin
- Pentanones
- Vanadium
- acetyl acetonate
|
Topics |
- Animals
- Bone and Bones
(metabolism)
- Cell Proliferation
- Chondrogenesis
(drug effects)
- Femoral Fractures
(therapy)
- Fracture Healing
(drug effects)
- Hydroxybutyrates
(pharmacology)
- Immunohistochemistry
(methods)
- Insulin
(metabolism)
- Male
- Neovascularization, Physiologic
(drug effects)
- Pentanones
(pharmacology)
- Rats
- Rats, Wistar
- Regeneration
- Stress, Mechanical
- Torque
- Vanadium
(pharmacology)
|