Investigations have characterized addictive drug-induced developmental cardiovascular malformation in human, non-human primate and rodent. However, the underlying mechanism of malformation caused by drugs during pregnancy is still largely unknown, and preventive and therapeutic measures have been lacking. Using 1H NMR spectroscopy, we profiled the metabolites from human embryo endothelial cells exposed to
methamphetamine (METH) and quantified a total of 226 peaks. We identified 11 metabolites modified robustly and found that
taurine markedly increased. We then validated the hypothesis that this dramatic increase in
taurine could attribute to its effect in inhibiting METH-induced developmental angiogenesis defect.
Taurine supplement showed a more significant potential than other metabolites in protecting against METH-induced injury in endothelial cells.
Taurine strongly attenuated METH-induced inhibition of proliferation and migration in endothelial cells. Furthermore, death rate and vessel abnormality of zebrafish embryos treated with METH were greatly reversed by
taurine. In addition,
taurine supplement caused a rapid decrease in
reactive oxygen species generation and strongly attenuated the excitable arise of antioxidase activities in the beginning of METH exposure prophase. Dysregulations of NF-κB, p-ERK as well as Bax, which reflect apoptosis, cell cycle arrest and oxidative stress in vascular endothelium, were blocked by
taurine. Our results provide the first evidence that
taurine prevents METH-caused developmental angiogenesis defect through
antioxidant mechanism.
Taurine could serve as a potential therapeutic or preventive intervention of developmental
vascular malformation for the pregnant women with drug use.