[Determination of melamine by high-performance liquid chromatography-mass spectrometry in urinary calculi].

Abstract

OBJECTIVE:

To establish a high-performance liquid chromatography-mass spectrometry (LC/MS)-based method for efficient determination of melamine in urinary calculi in children taking melamine-contaminated formula milk powder.

METHODS:

LC/MS was employed to determine the contents of melamine in urinary calculi surgically removed from 17 children with a history of taking melamine-contaminated milk powder and in 4 samples of uric acid stone from adults. The positive ionization mode of electro-spray ionization source was used, and the limit of melamine determination was 0.1 mg/kg.

RESULTS:

No melamine was detected in the 4 uric acid stone samples from adults. Melamine was detected in 4 samples of urinary calculi from the 17 children, with the concentration ranging from 0.8 to 64 mg/lkg.

CONCLUSION:

LC/MS is simple and effective for detecting melamine in urinary calculi, which is helpful to the treatment and follow-up.

• Authors: Wenqi Wu, Luping Wang, Lili Ou, Yeping Liang, Zanlin Mai, Shujue Li, Chichang Shan, Jian Yuan, Guohua Zeng
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 32 Issue 1 Pg. 125-7 (Jan 2012) ISSN: 1673-4254 [Print] China
• PMID: 22366022 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Triazines
• melamine

Topics

• Chromatography, High Pressure Liquid (methods)
• Dairy Products (adverse effects)
• Female
• Food Contamination
• Humans
• Infant
• Male
• Mass Spectrometry (methods)
• Triazines (analysis)
• Urinary Calculi (chemically induced, chemistry)