Mixed SAMs and MALDI-ToF MS: preparation of N-glycosylamine derivative and thioctic acid methyl ester bearing 1,2-dithiolane groups and detection of enzymatic reaction on Au.

Abstract

Herein, we report an enzymatic galactosylation reaction of β-glucopyranosylamide 4 and thioctic acid methyl ester 5 bearing 1,2-dithiolane groups to form a new system of mixed self-assembled monolayers (SAMs) on gold. Characterization of the enzymatic activity was conveniently achieved by mass spectrometry.

• Authors: Hani Mutlak A Hassan, Beatrice A Maltman
• Journal: Bioorganic chemistry (Bioorg Chem) Vol. 40 Issue 1 Pg. 6-9 (Feb 2012) ISSN: 1090-2120 [Electronic] United States
• PMID: 22130071 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Inc. All rights reserved.

Chemical References

• 1,2-dithiolane
• Amines
• Enzymes
• Thioctic Acid
• Gold
• Acetylglucosamine

Topics

• Acetylglucosamine (analogs & derivatives, chemical synthesis, chemistry)
• Amines (chemistry)
• Enzymes (metabolism)
• Gold (chemistry)
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Surface Properties
• Thioctic Acid (analogs & derivatives, chemical synthesis, chemistry)