Amelioration of immobilization stress-induced biochemical and behavioral alterations and mitochondrial dysfunction by naringin in mice: possible mechanism of nitric oxide modulation.

Abstract

OBJECTIVE:

The present study was undertaken to evaluate the effects of naringin on immobilization stress-induced biochemical-behavioral changes and mitochondrial dysfunction in mice.

METHODS:

Mice were randomized and grouped based on body weights. Respective drug treatments were given for 14 d, and on the 15th day all the animals were subjected to a 6-hour immobilization stress; then all the animals were subjected to various behavioral paradigms and were sacrificed. Various biochemical parameters and mitochondrial functions were analyzed using brain homogenate.

RESULTS:

The 6-hour acute immobilization stress significantly altered the behavioral (anxiety and memory) and biochemical parameters coupled with mitochondrial dysfunction in mice. Fourteen days pretreatment with naringin (50 and 100 mg/kg, per oral) significantly inhibited the behavioral and biochemical alterations and mitochondrial dysfunction caused by acute immobilization stress (P<0.05). Further, pretreatment with L-arginine (50 mg/kg, intraperitoneally), a nitric oxide precursor, reversed the protective effect of naringin (P<0.05). In addition, pretreatment with NG-nitro-L-arginine methyl ester (5 mg/kg, intraperitoneally) caused potentiation in the protective effect of naringin.

CONCLUSION:

These results suggest the possible involvement of nitrergic pathway in the protective effect of naringin against immobilization stress-induced behavioral, biochemical and mitochondrial dysfunctions in mice.

• Authors: Gollapalle L Viswanatha, Hanumanthappa Shylaja, K Sadashiva Sandeep Rao, Yathiraj Ashwini, V Ramaiah Santhosh Kumar, C Gangadharaiah Mohan, Venkate Gowda Sunil, M Venkateshappa Sarvesh Kumar, Subbanna Rajesh
• Journal: Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine (Zhong Xi Yi Jie He Xue Bao) Vol. 9 Issue 11 Pg. 1254-63 (Nov 2011) ISSN: 1672-1977 [Print] China
• PMID: 22088593 (Publication Type: Journal Article)

Chemical References

• Flavanones
• Nitric Oxide
• naringin

Topics

• Animals
• Behavior, Animal
• Brain (metabolism)
• Flavanones (pharmacology)
• Immobilization
• Lipid Peroxidation
• Male
• Mice
• Mitochondria (drug effects, metabolism)
• Nitric Oxide (metabolism)
• Oxidative Stress
• Stress, Physiological