[Clinical significance of large-scale screening of A1555G mutation of mitochondria DNA for neonates].

Abstract

OBJECTIVE:

To explore the necessity of large-scale screening of mitochondria DNA (mtDNA) A1555G mutation for prevention of aminoglycoside antibiotic induced deafness in newborns.

METHODS:

One thousand blood filter samples were collected from neonates born in July 2008 in Shenzhen. DNA was extracted with Chelex-100 Resin and amplified by PCR. The mtDNA A1555G mutation was determined by denaturing high-performance liquid chromatography(DHPLC) for PCR products. The positive frequency was calculated.

RESULTS:

The mitochondrial DNA A1555G mutation was detected in 2 cases of 1000 neonates. The frequency of mutation was 0.2%.

CONCLUSION:

There is a high frequency of mtDNA A1555G mutation in neonates, the large-scale screening of mtDNAA1555G mutation in newborns might detect the individuals sensitive to aminoglycoside antibiotic, which is helpful to guide a rational medication for newborns and the maternal relatives at high-risk. Furthermore, it might be useful to prevent aminoglycoside antibiotic induced deafness.

• Authors: Jun Cai, Cai-qun Luo, Jian-sheng Xie, Wei-qing Wu, Qian Geng, Zhi-yong Xu, Ying Hao, Xiao-xin Xu
• Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (Zhonghua Yi Xue Yi Chuan Xue Za Zhi) Vol. 28 Issue 4 Pg. 414-6 (Aug 2011) ISSN: 1003-9406 [Print] China
• PMID: 21811982 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Mitochondrial

Topics

• Base Sequence
• DNA Mutational Analysis (methods)
• DNA, Mitochondrial (genetics)
• Female
• Humans
• Infant, Newborn
• Male
• Polymerase Chain Reaction