Topical cream-based oxyresveratrol in the treatment of cutaneous HSV-1 infection in mice.

Abstract

Anti-herpes simplex virus (HSV) activities of oxyresveratrol in vitro and topical administration in cutaneous HSV-1 infection in mice were examined. The inhibitory concentrations for 50% plaque formation (IC(50)) of oxyresveratrol against HSV-1 clinical isolates and HSV-2 clinical isolates were 20.9-29.5 and 22.2-27.5 μg/ml, respectively. In topical administration in cutaneous HSV-1 infection in mice, 2.5%, 5%, 10% and 20% oxyresveratrol in cream vehicle applied three times daily for 7 days after infection were evaluated and 10% and 20% oxyresveratrol cream were significantly effective in delaying the development of skin lesions and protection from death (P < 0.01). The concentration of 10% oxyresveratrol in cream was significantly more effective than that of 30% oxyresveratrol in vaseline applied three times daily (P < 0.01). Oxyresveratrol cream at 20% was as effective as 5% ACV cream applied three times daily (P < 0.01). Both 10% and 20% oxyresveratrol cream were as effective as that of 5% ACV cream applied two times daily (P > 0.05). Therapeutic efficacy of oxyresveratrol in cream vehicle was dose-dependent and the maximum efficacy observed on day 6 after infection was shown at 10% oxyresveratrol in cream applied three times daily. The frequency of application of 10% oxyresveratrol cream at three, four and five times daily was as effective as that of 5% ACV cream applied five times daily (P > 0.05). These results demonstrated that topical administration of oxyresveratrol in novel cream vehicle reduced the concentration of oxyresveratrol to 10% and was suitable for cutaneous HSV infection.

• Authors: Vimolmas Lipipun, Pattaraporn Sasivimolphan, Yoshihiro Yoshida, Tohru Daikoku, Boonchoo Sritularak, Garnpimol Ritthidej, Kittisak Likhitwitayawuid, Pornpen Pramyothin, Masao Hattori, Kimiyasu Shiraki
• Journal: Antiviral research (Antiviral Res) Vol. 91 Issue 2 Pg. 154-60 (Aug 2011) ISSN: 1872-9096 [Electronic] Netherlands
• PMID: 21669230 (Publication Type: Journal Article)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Antiviral Agents
• Plant Extracts
• Stilbenes
• puag-haad
• Acyclovir

Topics

• Acyclovir (administration & dosage, therapeutic use)
• Administration, Cutaneous
• Animals
• Antiviral Agents (administration & dosage, chemistry, therapeutic use)
• Artocarpus (chemistry)
• Chlorocebus aethiops
• Dose-Response Relationship, Drug
• Female
• Herpes Simplex (drug therapy)
• Herpesvirus 1, Human (drug effects, growth & development)
• Herpesvirus 2, Human (drug effects, growth & development)
• Inhibitory Concentration 50
• Mice
• Mice, Inbred BALB C
• Microbial Sensitivity Tests (methods)
• Plant Extracts (administration & dosage, chemistry, therapeutic use)
• Skin Diseases, Infectious (drug therapy)
• Stilbenes (administration & dosage, chemistry, therapeutic use)
• Vero Cells
• Viral Plaque Assay