High expression of L-type amino-acid transporter 1 (LAT1) in gastric carcinomas: comparison with non-cancerous lesions.

Abstract

Amino acid transporters are essential for maintenance and proliferation of both normal and transformed cells. In the present study, L-type amino-acid transporter 1 (LAT1) immunoreactive expression was investigated in gastric carcinomas, in comparison with gastric adenomas and non-neoplastic lesions, using our recently developed novel monoclonal antibody. In a total of 87 cases of advanced gastric cancer, high LAT1 expression was observed in carcinoma cells, predominantly at plasma membranes with greater intensity in non-scirrhous than scirrhous carcinomas. Gastric carcinoma cases with lymph node metastasis showed significantly higher LAT1 expression than cases without lymph node metastasis. A positive correlation with Ki-67 LI was observed and the highly expressing non-scirrhous carcinomas showed a significantly poorer prognosis than the low LAT1 group. Cox hazard test revealed that TNM stage and LAT1 expression were independent prognostic factors in non-scirrhous carcinoma group. Further, a significant poor prognosis was confirmed in high LAT1 expression group, when limited to undifferentiated carcinoma cases excluding scirrhous carcinoma. Lower levels were found in adenomas. In conclusion, LAT 1 expression may be linked with cell proliferation and prognosis of gastric carcinomas, and offers a potential target for future anticancer therapy by inhibitors.

• Authors: Masaaki Ichinoe, Tetuo Mikami, Tsutomu Yoshida, Ikuyo Igawa, Tomoko Tsuruta, Norihiro Nakada, Naohiko Anzai, Yoshiyuki Suzuki, Hitoshi Endou, Isao Okayasu
• Journal: Pathology international (Pathol Int) Vol. 61 Issue 5 Pg. 281-9 (May 2011) ISSN: 1440-1827 [Electronic] Australia
• PMID: 21501294 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2011 The Authors. Pathology International © 2011 Japanese Society of Pathology and Blackwell Publishing Asia Pty Ltd.

Chemical References

• Antibodies, Monoclonal
• Biomarkers, Tumor
• Ki-67 Antigen
• Large Neutral Amino Acid-Transporter 1
• Tumor Suppressor Protein p53

Topics

• Adenocarcinoma (metabolism, mortality, pathology)
• Adenoma (metabolism, mortality, pathology)
• Adult
• Aged
• Aged, 80 and over
• Antibodies, Monoclonal
• Biomarkers, Tumor (metabolism)
• Cell Proliferation
• Female
• Gastric Mucosa (metabolism)
• Humans
• Immunohistochemistry
• Ki-67 Antigen (immunology, metabolism)
• Large Neutral Amino Acid-Transporter 1 (immunology, metabolism)
• Lymph Nodes (pathology)
• Lymphatic Metastasis
• Male
• Middle Aged
• Neoplasm Invasiveness
• Neoplasm Staging
• Precancerous Conditions (metabolism, mortality, pathology)
• Prognosis
• Proportional Hazards Models
• Stomach Neoplasms (metabolism, mortality, pathology)
• Tumor Suppressor Protein p53 (immunology, metabolism)