Abstract | PURPOSE: METHODS: Sixty-two pigs were studied. Either a pars plana intravitreal bevacizumab or a viscoelastic-enhanced microcannula suprachoroidal injection was performed with either 1.25 mg (group 1) or 3 mg (group 2). In group 1, six animals were euthanatized at 0.5, 7, 30, 60, 90, and 120 days after injection (n = 36). In group 2, six animals were euthanatized at 0.5, 7, 14, and 32 days (n = 24). Eyes were enucleated, dissected, and snap-frozen, or they were fixed for histology. Analysis of drug tissue levels was performed at two separate laboratories using masked specimens. RESULTS: Both laboratories were confirmatory. Intravitreal bevacizumab pharmacokinetics demonstrated a gradual decline in tissue levels over 30 to 60 days in both groups 1 and 2. In addition, suprachoroidal bevacizumab tissue levels declined rapidly and were not measurable at or beyond 7 days. Vitreitis and granulomatous vasculitis were noted in 7 of 30 intravitreal injection eyes. Immunohistology suggested a distinctive drug distribution. CONCLUSIONS:
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Authors | Timothy W Olsen, Xiao Feng, Kathy Wabner, Karl Csaky, Stefan Pambuccian, J Douglas Cameron |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 52
Issue 7
Pg. 4749-56
(Jul 01 2011)
ISSN: 1552-5783 [Electronic] United States |
PMID | 21447680
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Antibodies, Monoclonal, Humanized
- Bevacizumab
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Topics |
- Animals
- Antibodies, Monoclonal
(administration & dosage, pharmacokinetics)
- Antibodies, Monoclonal, Humanized
- Bevacizumab
- Catheterization
(methods)
- Choroid
(immunology, metabolism)
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Enzyme-Linked Immunosorbent Assay
- Follow-Up Studies
- Immunity, Cellular
(drug effects)
- Intravitreal Injections
- Macular Degeneration
(drug therapy, immunology, metabolism)
- Miniaturization
- Retinal Photoreceptor Cell Outer Segment
(immunology, metabolism)
- Retinal Pigment Epithelium
(immunology, metabolism)
- Swine
- Treatment Outcome
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