Cytotoxic T lymphocytes efficiently recognize human colon cancer stem-like cells.

Abstract

Cancer stem-like cells (CSCs) and tumor-initiating cells (TICs) are a small population of cancer cells that share three properties: tumor initiating ability, self-renewal, and differentiation. These properties suggest that CSCs/TICs are essential for tumor maintenance, recurrence, and distant metastasis. Here, we show that cytotoxic T lymphocytes (CTLs) specific for the tumor-associated antigen CEP55 can efficiently recognize colon CSCs/TICs both in vitro and in vivo. Using Hoechst 33342 dye staining, we isolated CSCs/TICs as side population (SP) cells from colon cancer cell lines SW480, HT29, and HCT15. The SP cells expressed high levels of the stem cell markers SOX2, POU5F1, LGR5, and ALDH1A1 and showed resistance to chemotherapeutic agents such as irinotecan or etoposide.To evaluate the susceptibility of SP cells to CTLs, we used CTL clone 41, which is specific for the CEP55-derived antigenic peptide Cep55/c10orf3_193 (10) (VYVKGLLAKI). The SP cells expressed HLA class I and CEP55 at the same level as the main population cells. The SP cells were susceptible to CTL clone 41 at the same level as main population cells. Furthermore, adoptive transfer of CTL clone 41 inhibited tumor growth of SW480 SP cells in vivo. These observations suggest that Cep55/c10orf3_193(10) peptide-based cancer vaccine therapy or adoptive cell transfer of the CTL clone is a possible approach for targeting chemotherapy-resistant colon CSCs/TICs.

• Authors: Satoko Inoda, Yoshihiko Hirohashi, Toshihiko Torigoe, Rena Morita, Akari Takahashi, Hiroko Asanuma, Munehide Nakatsugawa, Satoshi Nishizawa, Yasuaki Tamura, Tetsuhiro Tsuruma, Takeshi Terui, Toru Kondo, Kunihiko Ishitani, Tadashi Hasegawa, Koichi Hirata, Noriyuki Sato
• Journal: The American journal of pathology (Am J Pathol) Vol. 178 Issue 4 Pg. 1805-13 (Apr 2011) ISSN: 1525-2191 [Electronic] United States
• PMID: 21435460 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

Chemical References

• Antigens
• Antineoplastic Agents
• Benzimidazoles
• Peptides
• Etoposide
• Irinotecan
• bisbenzimide ethoxide trihydrochloride
• Camptothecin

Topics

• Animals
• Antigens (chemistry)
• Antineoplastic Agents (pharmacology)
• Benzimidazoles (pharmacology)
• Camptothecin (analogs & derivatives, pharmacology)
• Cell Line, Tumor
• Colonic Neoplasms (immunology)
• Etoposide (pharmacology)
• Humans
• Irinotecan
• Mice
• Mice, Inbred NOD
• Mice, SCID
• Neoplasm Transplantation
• Neoplastic Stem Cells (immunology)
• Peptides (chemistry)
• T-Lymphocytes, Cytotoxic (cytology)