Effect of normobaric oxygen therapy in a rat model of intracerebral hemorrhage.

Abstract

BACKGROUND AND PURPOSE:

Normobaric oxygen (NBO) therapy may be neuroprotective in acute ischemic stroke. However, how NBO may affect intracerebral hemorrhage is unclear. We tested NBO in a rat model of striatal intracerebral hemorrhage.

METHODS:

Intracerebral hemorrhage was induced by stereotactic injection of collagenase Type VII (0.5 U) into the right striatum of male Sprague-Dawley rats. One hour later, rats were randomized into controls (n=13) versus NBO treatment (n=13). NBO was applied for 2 hours. Hemorrhagic blood volume, brain water content, and neurological outcomes (forelimb placement test, forelimb asymmetry, neuroscore) were quantified at 72 hours. Experiments were repeated in a second independent laboratory to assess reproducibility in neurological outcomes (n=10 per group).

RESULTS:

NBO did not worsen hemorrhage severity or brain edema. There were no significant differences in hemorrhagic blood volumes (control, 6.4±0.9 μL versus NBO, 7.0±2.1 μL; P=0.18) or brain water content (control, 81.9%±1.1% versus NBO, 81.6%±0.5%; P=0.58). NBO did not affect any of the neurological outcome tests in the primary or secondary studies.

CONCLUSIONS:

NBO therapy may not worsen outcomes in intracerebral hemorrhage.

• Authors: Norio Fujiwara, Emiri T Mandeville, Xiaokun Geng, Yumin Luo, Ken Arai, Xiaoying Wang, Xunming Ji, Aneesh B Singhal, Eng H Lo
• Journal: Stroke (Stroke) Vol. 42 Issue 5 Pg. 1469-72 (May 2011) ISSN: 1524-4628 [Electronic] United States
• PMID: 21415401 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Water
• Oxygen

Topics

• Animals
• Blood Volume (drug effects)
• Brain (drug effects, metabolism)
• Cerebral Hemorrhage (metabolism, therapy)
• Male
• Models, Animal
• Oxygen (pharmacology)
• Oxygen Inhalation Therapy (methods)
• Rats
• Rats, Sprague-Dawley
• Treatment Outcome
• Water (metabolism)