A novel Glycine soja tonoplast intrinsic protein gene responds to abiotic stress and depresses salt and dehydration tolerance in transgenic Arabidopsis thaliana.

Abstract

Tonoplast intrinsic protein (TIP) is a subfamily of the aquaporin (AQP), also known as major intrinsic protein (MIP) family, and regulates water movement across vacuolar membranes. Some reports have implied that TIP genes are associated with plant tolerance to some abiotic stresses that cause water loss, such as drought and high salinity. In our previous work, we found that an expressed sequence tag (EST) representing a TIP gene in our Glycine soja EST library was inducible by abiotic stresses. This TIP was subsequently isolated from G. soja with cDNA library screening, EST assembly and PCR, and named as GsTIP2;1. The expression patterns of GsTIP2;1 in G. soja under low temperature, salt and dehydration stress were different in leaves and roots. Though GsTIP2;1 is a stress-induced gene, overexpression of GsTIP2;1 in Arabidopsis thaliana depressed tolerance to salt and dehydration stress, but did not affect seedling growth under cold or favorable conditions. Higher dehydration speed was detected in Arabidopsis plants overexpressing GsTIP2;1, implying GsTIP2;1 might mediate stress sensitivity by enhancing water loss in the plant. Such a result is not identical to previous reports, providing some new information about the relationship between TIP and plant abiotic stress tolerance.

• Authors: Xi Wang, Yong Li, Wei Ji, Xi Bai, Hua Cai, Dan Zhu, Xiao-Li Sun, Lian-Jiang Chen, Yan-Ming Zhu
• Journal: Journal of plant physiology (J Plant Physiol) Vol. 168 Issue 11 Pg. 1241-8 (Jul 15 2011) ISSN: 1618-1328 [Electronic] Germany
• PMID: 21397356 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier GmbH. All rights reserved.

Chemical References

• Arabidopsis Proteins
• Membrane Proteins
• Plant Proteins
• Soybean Proteins
• tonoplast intrinsic protein, plant
• Water
• Sodium Chloride
• Acyltransferases
• TIP1 protein, Arabidopsis

Topics

• Acyltransferases (genetics, metabolism)
• Arabidopsis (genetics, metabolism, physiology)
• Arabidopsis Proteins (genetics, metabolism)
• Cold Temperature
• Droughts
• Expressed Sequence Tags
• Gene Expression Regulation, Plant
• Gene Library
• Genes, Plant
• Membrane Proteins (genetics, metabolism)
• Phylogeny
• Plant Leaves (genetics, metabolism, physiology)
• Plant Proteins (genetics, metabolism)
• Plant Roots (genetics, metabolism, physiology)
• Plants, Genetically Modified (genetics, metabolism, physiology)
• Salt-Tolerant Plants (genetics, metabolism, physiology)
• Seedlings (genetics, metabolism, physiology)
• Sequence Homology, Amino Acid
• Sodium Chloride (metabolism)
• Soybean Proteins (genetics, metabolism)
• Glycine max (genetics, metabolism, physiology)
• Stress, Physiological
• Water (metabolism)