[Effect of baicalin on protease-activated receptor-1 expression and cell apoptosis in brain of rat with intracerebral hemorrhage].

Abstract

OBJECTIVE:

To investigate the protective effect and mechanism of baicalin on nerve tissue in rat with intracerebral hemorrhage (ICH).

METHODS:

Rats were randomly divided into five groups: the sham-operated group, the ICH model group, and the three baicalin treated groups treated respectively with small, medium and large doses of baicalin. ICH rat model was established by injecting collagenase VII into caudate nucleus. Baicalin was given by peritoneal injection to the baicalin treated groups, and saline was given to the other two groups once a day started from 2 h after modeling. Animals were sacrificed in batches on the 1st, 3rd, 5th and 10th day of treatment to take their brains for detecting protease-activated receptor-1 (PAR-1) expression and cell apoptosis in brain tissue surrounding hematoma by Western blot and TUNEL method, respectively. And the water content of brain was estimated by dry-wet weight method.

RESULTS:

Compared with the model group, the PAR-1 expression and TUNEL-positive cells were significantly reduced in the baicalin treated groups; and brain edema was also significantly reduced (P<0.01).

CONCLUSIONS:

The up-regulated PAR-1 expression after ICH in rats might play an important role in inducing cell apoptosis and brain edema. Baicalin shows significant protective effect on ICH rats, which may be related to its effects in inhibiting PAR-1 expression and decreasing apoptosis cells, so as to reduce brain edema.

• Authors: Qing-bo Zhou, Qing Jia, Yuan Zhang
• Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine (Zhongguo Zhong Xi Yi Jie He Za Zhi) Vol. 30 Issue 12 Pg. 1302-5 (Dec 2010) ISSN: 1003-5370 [Print] China
• PMID: 21302496 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Flavonoids
• Receptor, PAR-1
• baicalin

Topics

• Animals
• Apoptosis (drug effects)
• Brain (metabolism, pathology)
• Cerebral Hemorrhage (drug therapy)
• Depression, Chemical
• Flavonoids (pharmacology, therapeutic use)
• Male
• Phytotherapy
• Rats
• Rats, Wistar
• Receptor, PAR-1 (metabolism)