Roles of the ribosomal protein S19 dimer and chemically induced apoptotic cells as a tumor vaccine in syngeneic mouse transplantation models.
|
| Abstract |
We examined the roles of a chemotherapeutic reagent in both inducing apoptosis and conferring acquired tumor immunity using mouse syngeneic tumor transplantation models. A chemotherapeutic reagent, cis-diamminedichloroplatinum (II), effectively induced apoptosis in Colon-26 cells originating from a BALB/c mouse. Three intradermal inoculations of cis-diamminedichloroplatinum (II)-treated Colon-26 cells conferred tumor immunity against viable Colon-26 cells in BALB/c mice but not in athymic BALB/c mice. The acquired immunity was Colon-26 cell specific and was adoptively transferable with splenic cells. We next examined the involvement of the cross-linked ribosomal protein S19 dimer (RP S19), which is released during apoptosis, in the acquisition of tumor immunity. A Colon-26 transformant that produces a Gln137Asn-mutant RP S19 prevents the formation of a functional RP S19 dimer. Acquired tumor immunity was significantly reduced when the transformant was used in combination with anti-RP S19 antibodies as the vaccination source. These data suggest the importance of the RP S19 dimer in chemotherapeutic agent-induced acquired immunity.
|
|---|---|
| Authors | Keisuke Taniguchi, Hiroshi Nishiura, Yoshihiko Ota, Tetsuro Yamamoto |
| Journal | Journal of immunotherapy (Hagerstown, Md. : 1997) (J Immunother) Vol. 34 Issue 1 Pg. 16-27 (Jan 2011) ISSN: 1537-4513 [Electronic] United States |
| PMID | 21150710 (Publication Type: Journal Article) |
| Chemical References |
|
| Topics |
|
Join CureHunter, for free Research Interface BASIC access!
Realize the full power of the drug-disease research graph!