A strategy for detecting optimal ratio of cardioprotection-dependent three compounds as quality control of guan-xin-er-hao formula.

Abstract

AIMS:

We aimed to detect optimal ratio of cardioprotection-dependent absorbed bioactive compounds (ABCs) as quality control of guan-xin-er-hao (GXEH) formula extracted by various processings.

METHODS:

Ferulic acid (F), tanshinol (T), hydroxysafflor yellow A (A), protocatechualdehyde (P) and paeoniflorin (E) in GXEH formula and FTA in blood from rat with acute myocardial infarction (AMI) were first identified by HPLC-MS/MS, and FTAPE in GXEH formulae with various herbs, extraction times and extraction water volumes were then quantitated only by HPLC.

RESULTS:

FTAPE in various GXEH were determined. FTA were selected as GXEH's ABCs. Ratios of FTA were determined, suggesting the high (1:6.1:15.6), medium (1:1.7:15.2) and low (1:0.2:15.3) ratios. Three FTA ratios and their parent formulae ratio-dependently reduced infarct size, myocardial apoptosis and caspase-3 activity.

CONCLUSION:

There is the optimal ratio of F:T:A among various formulae, contributing to the best cardioprotection. This FTA ratio was developed as quality control of GXEH formula.

• Authors: Yang Wang, Xi Huang, Feng Qin, Ping Ren, Zhengyan Zhu, Rong Fan, Chunhu Zhang, Hainan Zhang, Tao Tang, Juan He, Zhaoqian Liu, Honghao Zhou
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 133 Issue 2 Pg. 735-42 (Jan 27 2011) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 21073938 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Crown Copyright © 2010. Published by Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Benzaldehydes
• Benzoates
• Bridged-Ring Compounds
• Cardiotonic Agents
• Catechols
• Coumaric Acids
• Drugs, Chinese Herbal
• Glucosides
• Monoterpenes
• Quinones
• guan xin II
• hydroxysafflor yellow A
• peoniflorin
• protocatechualdehyde
• Chalcone
• ferulic acid
• Casp3 protein, rat
• Caspase 3

Topics

• Animals
• Apoptosis (drug effects)
• Benzaldehydes (administration & dosage)
• Benzoates (administration & dosage)
• Bridged-Ring Compounds (administration & dosage)
• Cardiotonic Agents (administration & dosage, chemistry, standards)
• Caspase 3 (metabolism)
• Catechols (administration & dosage)
• Chalcone (administration & dosage, analogs & derivatives)
• Chemistry, Pharmaceutical
• Coumaric Acids (administration & dosage)
• Drugs, Chinese Herbal (administration & dosage, chemistry, pharmacokinetics, standards)
• Ethnopharmacology
• Glucosides (administration & dosage)
• Male
• Monoterpenes
• Myocardial Infarction (drug therapy, metabolism, pathology)
• Phytotherapy
• Quality Control
• Quinones (administration & dosage)
• Rats
• Rats, Sprague-Dawley