Abstract | BACKGROUND AND OBJECTIVE: METHODS: The proliferation of lung adenocarcinoma A549 cells was detected by MTT. The apoptosis rate was observed with MTT assay and flow cytometer. The activity of catalase was detected by colorimetry. The malondialdehyde (MDA) content was determined by improved thiobarbituric acid fluorometric method. The expression of P38MAPK protein was analyzed with Western blot. RESULTS:
Spider venom can remarkably inhibite the proliferation of lung adenocarcinoma A549 cells, increased activity of catalase and MDA content, down-regulated expression of P38MAPK compared with the control group. CONCLUSIONS:
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Authors | Zengxiang Hu, Yulei Du, Quanxi Liu, Yuan Wang, Liang Li |
Journal | Zhongguo fei ai za zhi = Chinese journal of lung cancer
(Zhongguo Fei Ai Za Zhi)
Vol. 13
Issue 10
Pg. 933-6
(Oct 2010)
ISSN: 1999-6187 [Electronic] China |
PMID | 20959064
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Spider Venoms
- Malondialdehyde
- Catalase
- p38 Mitogen-Activated Protein Kinases
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Topics |
- Adenocarcinoma
(enzymology, genetics, metabolism, pathology)
- Adenocarcinoma of Lung
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Catalase
(metabolism)
- Cell Division
(drug effects)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- G2 Phase
(drug effects)
- Gene Expression Regulation, Neoplastic
(drug effects)
- Humans
- Lung Neoplasms
(enzymology, genetics, metabolism, pathology)
- Malondialdehyde
(metabolism)
- Spider Venoms
(pharmacology)
- p38 Mitogen-Activated Protein Kinases
(metabolism)
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